Key Product Details

Species Reactivity

Validated:

Chicken

Cited:

Avian - Chicken

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RGM-A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant chicken RGM-A
Trp30-Asn404 (Asn404Asp)
Accession # Q8JG54

Specificity

Detects chicken RGM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross‑reactivity with recombinant human RGM and recombinant mouse RGM.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Chicken RGM‑A Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Chicken Repulsive Guidance Molecule A/RGM-A

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RGM-A

Chicken RGM is the founding member of the RGM family of glycosylphosphatidylinositol (GPI)-anchored axon guidance proteins. It is a disulfide-linked dimer of the N-terminal RGD-containing segment (aa 30‑149) and the C-terminal segment (aa 150‑432). RGM-A binds with high affinity to neogenin. Chicken RGM-A (aa 30‑401) shares 88% and 87% aa sequence identity with its human and mouse homologs, respectively.

Long Name

Repulsive Guidance Molecule A

Alternate Names

RGMA

Entrez Gene IDs

56963 (Human); 244058 (Mouse)

Gene Symbol

RGMA

UniProt

Q8JG54

Additional RGM-A Products

Product Documents for Chicken RGM‑A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Chicken RGM‑A Antibody

For research use only

Related Research Areas

BMP Family

Citations for Chicken RGM‑A Antibody

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