Chitinase 3-like 2 Antibody (2B5) - BSA Free

Novus Biologicals | Catalog # NBP2-60238

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2B5

Format

BSA Free
View all Chitinase 3-like 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Chitinase 3-like 2 (27-390aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for Chitinase 3-like 2 Antibody (2B5) - BSA Free

Western Blot: Chitinase 3-like 2 Antibody (2B5)BSA Free [NBP2-60238]

Western Blot: Chitinase 3-like 2 Antibody (2B5)BSA Free [NBP2-60238]

Western Blot: Chitinase 3-like 2 Antibody (2B5) [NBP2-60238] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Chitinase 3-like 2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: HeLa cell lysate Lane 3.: HepG2 cell lysate Lane 4.: LnCap cell lysate
Flow Cytometry: Chitinase 3-like 2 Antibody (2B5) - BSA Free [NBP2-60238]

Flow Cytometry: Chitinase 3-like 2 Antibody (2B5) - BSA Free [NBP2-60238]

Flow Cytometry: Chitinase 3-like 2 Antibody (2B5) [NBP2-60238] - Analysis of CHI3L2 in HeLa cells. The cell was stained with at 2-5ug for 1x106cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).

Applications for Chitinase 3-like 2 Antibody (2B5) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

2-5 ug

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Chitinase 3-like 2

CHI3L2 may bind glycan structure with high affinity, but not heparin. Has no chitotriosidase activity

Alternate Names

CHI3L2, Chitinase 3 like 2, YKL39

Gene Symbol

CHI3L2

Additional Chitinase 3-like 2 Products

Product Documents for Chitinase 3-like 2 Antibody (2B5) - BSA Free

Certificate of Analysis

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Product Specific Notices for Chitinase 3-like 2 Antibody (2B5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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