Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Chk1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residues 200 and 250 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Chk1 Antibody - BSA Free

Western Blot: Chk1 Antibody [NB100-274]

Western Blot: Chk1 Antibody [NB100-274]

Western Blot: Chk1 Antibody [NB100-274] - Samples: Whole cell lysate (50 ug) from 293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-Chk1 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Chk1 Antibody [NB100-274]

Western Blot: Chk1 Antibody [NB100-274]

Western Blot: Chk1 Antibody [NB100-274] - Whole cell lysate (30 ug) from HeLa cells treated with 10 Gy ionizing radiation (IR) or untreated. Antibody used at 0.1 ug/ml.

Applications for Chk1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:2500-1:15000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Chk1

In response to DNA damage and replication blocks, cells prevent cell cycle progression through the control of critical cell cycle regulators. Chk1 is an essential kinase for embryonic development and regulating mitotic progression in response to DNA damage.

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Entrez Gene IDs

1111 (Human)

Gene Symbol

CHEK1

UniProt

O14757

Additional Chk1 Products

Product Documents for Chk1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Chk1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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