Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60038

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, urine and cell culture supernates (50 - 100 uL)

Sensitivity

2 mIU/mL

Assay Range

8 mIU/mL - 240 mIU/mL
View all Chorionic Gonadotropin beta Chain (HCG beta) ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human HCG. No significant cross-reactivity or interference between human HCG and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 15

Inter-Assay Precision (Precision between assays) %CV 15

Scientific Data Images

ELISA: Human hCG ELISA Kit (Colorimetric) [NBP2-60038]
Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric)

Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric) [NBP2-60038] -

Human Chorionic Gonadotropin beta Chain (hCG beta) ELISA Kit (Colorimetric) [NBP2-60038] - Standard Curve Reference

Kit Contents

  • Adhesive Strip (For 96 wells)
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Sample Diluent (for urine only, 10 x concentrate)
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Chorionic Gonadotropin beta Chain (HCG beta)

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HCG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for HCG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HCG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Chorionic Gonadotropin beta Chain

Alternate Names

CG-Beta, CGB, CGB3, CGB5, CGB8, Choriogonadotropin Subunit Beta

Gene Symbol

CGB3

Additional Chorionic Gonadotropin beta Chain (HCG beta) Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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