Chromogranin A Antibody (CHGA/798) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48072

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CHGA/798

Format

IHC-Prediluted
View all Chromogranin A Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Chromogranin A protein (Uniprot: P10645)

Localization

Finely granular cytoplasmic

Marker

Neuroendocrine Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Chromogranin A Antibody (CHGA/798) - IHC-Prediluted

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072] - Human SC Lung Carcinoma stained with Chromogranin A MAb (CHGA/798)
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072] - Mouse Pancreas stained with Chromogranin A Antibody (CHGA/798).
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072] - Formalin-paraffin Rat Pancreas stained with Chromogranin A MAb (CHGA/798)
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072] - Human Parathyroid stained with Chromogranin A Monoclonal Antibody (CHGA/798)
Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072] - Human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (CHGA/798)
Chromogranin A Antibody (CHGA/798) - IHC-Prediluted Protein Array: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072] -

Protein Array: Chromogranin A Antibody (CHGA/798) - IHC-Prediluted [NBP2-48072] -

Analysis of Protein Array containing more than 19,000 full-length human proteins using Chromogranin A Antibody (CHGA/798) - IHC-Prediluted Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Applications for Chromogranin A Antibody (CHGA/798) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Chromogranin A

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (CHGA/798) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Chromogranin A Antibody (CHGA/798) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Chromogranin A Antibody (CHGA/798) - IHC-Prediluted

There are currently no reviews for this product. Be the first to review Chromogranin A Antibody (CHGA/798) - IHC-Prediluted and earn rewards!

Have you used Chromogranin A Antibody (CHGA/798) - IHC-Prediluted?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Chromogranin A Antibody (CHGA/798) - IHC-Prediluted

Showing  1 - 1 of 1 FAQ Showing All

  • Q: I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?

    A:

    When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies