Chromogranin A Antibody (LK2H10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-34672

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Guinea Pig (Negative), Monkey, Rabbit (Negative), Sheep (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LK2H10

Format

Azide and BSA Free
View all Chromogranin A Primary Antibodies »

Product Specifications

Immunogen

Human pheochromocytoma (Uniprot: P10645)

Reactivity Notes

Does not react with Guinea Pig, Sheep or Rabbit.

Localization

Finely granular cytoplasmic

Marker

Neuroendocrine Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32956).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Chromogranin A Antibody (LK2H10) - Azide and BSA Free

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672] - Human Pancreas FFPE slide: immunofluorescence with Chromogranin A Antibody-LK2H10 as primary antibody and CF405M as secondary antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672] - Mouse Pancreas stained with Chromogranin A Antibody (LK2H10).
Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with Chromogranin A Monoclonal Antibody (LK2H10)
Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672]

Immunohistochemistry-Paraffin: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672] - Formalin-fixed, paraffin-embedded human small cell lung carcinoma stained with Chromogranin A MAb (LK2H10)
Chromogranin A Antibody (LK2H10) - Azide and BSA Free Western Blot: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672] -

Western Blot: Chromogranin A Antibody (LK2H10) - Azide and BSA Free [NBP2-34672] -

Western blot analysis of human Pancreas tissue lysate using Chromogranin A Antibody (LK2H10) - Azide and BSA Free.

Applications for Chromogranin A Antibody (LK2H10) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Reviewed Applications

Read 1 review rated 5 using NBP2-34672 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Chromogranin A

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (LK2H10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Chromogranin A Antibody (LK2H10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Chromogranin A Antibody (LK2H10) - Azide and BSA Free

Customer Reviews for Chromogranin A Antibody (LK2H10) - Azide and BSA Free (1)

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Customer Images

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  • Chromogranin A Antibody (LK2H10)
    Name: Anonymous
    Application: Immunofluorescence-Paraffin
    Sample Tested: FFPE slide
    Species: Human Pancreas
    Verified Customer | Posted 09/30/2022
    Human Pancreas FFPE slide: immunofluorescence with Chromogranin A Antibody-LK2H10 as primary antibody and CF405M as 2nd antibody.
    Chromogranin A Antibody (LK2H10) - Azide and BSA Free NBP2-34672

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Protocols

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FAQs for Chromogranin A Antibody (LK2H10) - Azide and BSA Free

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  • Q: I have following question about antibodies against Chromogranin A. Your products list the species but canine is not included. Does it mean that the products cannot be used on canine tissues?

    A:

    When we do not list a species, it means we have not tested an antibody to work with those samples. If we test an antibody and find that it does not work, we will list the species with a minus sign. Unfortunately, we haven't tried these abs on canine tissues and they may work, but we cannot guarantee it. If you would like to purchase one to try, you will be eligible for the Innovators Reward Program.

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