CIZ1 Antibody

Novus Biologicals | Catalog # NBP2-26207

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CIZ1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-QPNTQDKTPSKVTAR corresponding to C-Terminus according to NP_036259.2, NP_001124487.1, NP_001124489.1, NP_001124490.1, NP_001244904.1, NP_001244905.1.

Specificity

This antibody is expected to recognize all reported isoforms (NP_036259.2; NP_001124487.1; NP_001124489.1; NP_001124490.1; NP_001244904.1; NP_001244905.1). Reported variants represent identical protein: NP_036259.2, NP_001124488.1

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CIZ1 Antibody

Western Blot: CIZ1 Antibody [NBP2-26207]

Western Blot: CIZ1 Antibody [NBP2-26207]

Western Blot: CIZ1 Antibody [NBP2-26207] - Staining of HeLa nuclear lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for CIZ1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 90 kDa band observed in HeLa nuclear lysates (calculated MW of 91.2 kDa band according to NP_001124490.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CIZ1

CIZ1 (Cip1 interacting zinc-finger protein) was identified as a protein that associates with the cell-cycle inhibiting protein, p21 (Cip1/Waf1). CIZ1 is a member of the matrin 3 protein family and has been shown to bind DNA consensus sequences and co-localize with PCNA during replication. In breast cancer cells, CIZ1 was shown to associate with Cdk2 and dynein light chain 1 (DLC1), a cytoskeletal signaling protein involved in cell cycle regulation and tumorigenesis. Studies of the role of CIZ1 in breast tumorigenesis revealed that CIZ1 is an estrogen-responsive gene that functions as a coactivator of the estrogen receptor. Targeted depletion of CIZ1 inhibits entry into S-phase. This finding along with evidence that CIZ1 interacts with multiple cell cycle regulatory proteins and localizes to DNA replication foci, indicates that CIZ1 plays a critical role in the regulation of DNA replication.

Alternate Names

CDKN1A interacting zinc finger protein 1, CDKN1A-interacting zinc finger protein 1, cip1-interacting zinc finger protein, LSFR1ZNF356Nuclear protein NP94, NP94, Zinc finger protein 356

Entrez Gene IDs

25792 (Human)

Gene Symbol

CIZ1

Additional CIZ1 Products

Product Documents for CIZ1 Antibody

Certificate of Analysis

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Product Specific Notices for CIZ1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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