CKMT2 Antibody - BSA Free

Western Blot: CKMT2 Antibody - BSA Free [NBP2-13841] -

BMP7 treatment upregulated creatine futile cycle-mediated thermogenesis in SC and DN derived differentiated adipocytes. SC and DN preadipocytes were differentiated and treated as in Figure 1, Figure 2, Figure 3, Figure 4 and Figure 5. (A) Representative time lapse OCR curve after indicated treatments, followed by quantification of creatine cycle related ( beta -GPA inhibited) OCR (n = 5). (B) Quantification of gene expression of CKMT2 by RNA-sequencing (n = 9) and RT-qPCR (n = 5), followed by protein expression (n = 5). (C) Quantification of gene expression of CKB by RNA-sequencing (n = 9), followed by protein expression (n = 4). (D) Quantification of gene expression of TNAP by RNA-sequencing (n = 9). Data expressed as mean +/- SD. RT-qPCR was normalized to GAPDH, protein expression was normalized to beta -actin. * p < 0.05, ** p < 0.01. Statistics: one-way ANOVA with Tukey’s post-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34832860), licensed under a CC-BY license. Not internally tested by Novus Biologicals.