Claudin-1 Antibody (CL3698) - Azide and BSA Free

Name: Claudin-1 Antibody (CL3698) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-43958
Price: 539 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-43958

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL3698

Format

Azide and BSA Free

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Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of O95832, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Claudin-1 Antibody (CL3698) - Azide and BSA Free

Western Blot: Claudin-1 Antibody [NBP3-43958]

Analysis in human cell lines RT-4 and U-251MG using Anti-Claudin-1 antibody. Corresponding Claudin-1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.

Applications for Claudin-1 Antibody (CL3698) - Azide and BSA Free

Application

Recommended Usage

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Claudin-1

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Alternate Names

Claudin1, CLD1, CLDN1, SEMP1

Gene Symbol

CLDN1

Additional Claudin-1 Products

Product Documents for Claudin-1 Antibody (CL3698) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for Claudin-1 Antibody (CL3698) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.