![Immunohistochemistry-Paraffin: CLEC3A Antibody [NBP2-30595]](https://resources.rndsystems.com/images/products/CLEC3A-Antibody-Immunohistochemistry-Paraffin-NBP2-30595-img0003.jpg "Immunohistochemistry-Paraffin: CLEC3A Antibody [NBP2-30595]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: DCISKGGILVIPRNSDEINALQDYGKRSLPGVNDFWLGINDMVTEGKFVDVNGIAISFLNWDR
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (87%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit CLEC3A Antibody - BSA Free (NBP2-30595) is a polyclonal antibody validated for use in IHC and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CLEC3A Antibody - BSA Free
Immunohistochemistry-Paraffin: CLEC3A Antibody [NBP2-30595]
Immunohistochemistry-Paraffin: CLEC3A Antibody [NBP2-30595] - Staining of human cerebral cortex shows strong cytoplasmic positivity, with a granular pattern in neuronal cells.![Immunofluorescence: CLEC3A Antibody [NBP2-30595]](https://resources.rndsystems.com/images/products/CLEC3A-Antibody-Immunofluorescence-NBP2-30595-img0004.jpg "Immunofluorescence: CLEC3A Antibody [NBP2-30595]")
Immunofluorescence: CLEC3A Antibody [NBP2-30595]
Immunofluorescence: CLEC3A Antibody [NBP2-30595] - Human tonsil was stained for Clec3A (red) and counterstained with DAPI. This image was submitted via customer review.
Immunohistochemistry-Paraffin: CLEC3A Antibody [NBP2-30595] -
Immunohistochemistry-Paraffin: CLEC3A Antibody [NBP2-30595] -Staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: CLEC3A Antibody [NBP2-30595] -
Immunohistochemistry-Paraffin: CLEC3A Antibody [NBP2-30595] -Staining of human cerebellum shows strong granular pattern cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: CLEC3A Antibody [NBP2-30595] -
Immunohistochemistry-Paraffin: CLEC3A Antibody [NBP2-30595] -Staining of human skin show negative cytoplasmic positivity in fibroblasts as expected.Applications for CLEC3A Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Validated from a verified customer review
Immunohistochemistry
1:5000 - 1:10000
Immunohistochemistry-Paraffin
1:5000 - 1:10000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Reviewed Applications
Read 1 review rated 4 using NBP2-30595 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: CLEC3A
Long Name
C-Type Lectin Domain Family 3 Member A
Alternate Names
Cartilage-Derived C-Type Lectin, CLECSF1
Gene Symbol
CLEC3A
UniProt
Additional CLEC3A Products
Product Documents for CLEC3A Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CLEC3A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for CLEC3A Antibody - BSA Free (1)
4 out of 5
1 Customer Rating
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Application: ImmunofluorescenceSample Tested: Human Tonsil tissueSpecies: HumanVerified Customer | Posted 02/02/2018Human tonsil was stained for Clec3A (red) and counterstained with DAPIAcetone fixation blocking with Fc blocker and background buster satining 1h incubation at room temp.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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