CLOCK Antibody (HL1099) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13718

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1099 expressed in HEK293

Format

Azide and BSA Free
View all CLOCK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human CLOCK. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for CLOCK Antibody (HL1099) - Azide and BSA Free

Western Blot: CLOCK Antibody (HL1099) [NBP3-13718]

Western Blot: CLOCK Antibody (HL1099) [NBP3-13718]

Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Untreated (-) and treated (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
CLOCK Antibody (HL1099)

Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (HL1099) [NBP3-13718] -

Immunocytochemistry/ Immunofluorescence: CLOCK Antibody (HL1099) [NBP3-13718] - CLOCK antibody [HL1099] detects CLOCK protein by immunofluorescent analysis.
Sample: DIV9 rat hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CLOCK stained by CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:500.
Red: Tau, a Axon marker, stained by Tau antibody [GT287] diluted at 1:500.
Blue: Fluoroshield with DAPI.
CLOCK Antibody (HL1099)

Immunohistochemistry-Paraffin: CLOCK Antibody (HL1099) [NBP3-13718] -

Immunohistochemistry-Paraffin: CLOCK Antibody (HL1099) [NBP3-13718] - CLOCK antibody [HL1099] detects CLOCK protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
CLOCK stained by CLOCK antibody [HL1099] (NBP3-13718) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CLOCK Antibody (HL1099)

Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] -

Western Blot: CLOCK Antibody (HL1099) [NBP3-13718] - Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CLOCK antibody [HL1099] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for CLOCK Antibody (HL1099) - Azide and BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLOCK

Circadian Locomotor Output Cycles Kaput (CLOCK) is a bHLH/PAS protein that plays an important role in the molecular clock mechanism. Specifically, CLOCK activates the central circadian loop by stimulating expression of Period and Timeless proteins. CLOCK has also been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.

CLOCK levels peak during the late night and the early part of the subjective day. Mutations in CLOCK causes defective transcriptional activity and lengthens the circadian period, leading to abnormal circadian behavior.

CLOCK antibodies are useful tools for research on circadian rythmns, sleep biology and hormonal cycles.

Long Name

Circadian Locomoter Output Cycles Kaput

Alternate Names

BHLHE8, circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, clock (mouse) homolog, clock homolog (mouse), EC 2.3.1.48, hCLOCK, KAT13D, KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Gene Symbol

CLOCK

Additional CLOCK Products

Product Documents for CLOCK Antibody (HL1099) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CLOCK Antibody (HL1099) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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