CNAP1 Antibody (4C12) - Azide and BSA Free

Novus Biologicals | Catalog # H00009918-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4C12

Format

Azide and BSA Free
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Product Specifications

Immunogen

CNAP1 (AAH28182, 1240 a.a. ~ 1339 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LRKMLDNFDCFGDKLSDESIFSAFLSVVGKLRRGAKPEGKAIIDEFEQKLRACHTRGLDGIKELEIGQAGSQRAPSAKKPSSGSRYQPLASTASDNDFVT

Reactivity Notes

Human. Other species not tested.

Localization

Nuclear and cytoplasmic.

Specificity

CNAP1 - chromosome condensation-related SMC-associated protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CNAP1 Antibody (4C12) - Azide and BSA Free

Western Blot: CNAP1 Antibody (4C12) [H00009918-M01]

Western Blot: CNAP1 Antibody (4C12) [H00009918-M01]

Western Blot: CNAP1 Antibody (4C12) [H00009918-M01] - CNAP1 monoclonal antibody (M01), clone 4C12 Analysis of CNAP1 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: CNAP1 Antibody (4C12) [H00009918-M01]

Immunocytochemistry/ Immunofluorescence: CNAP1 Antibody (4C12) [H00009918-M01]

Immunocytochemistry/Immunofluorescence: CNAP1 Antibody (4C12) [H00009918-M01] - Analysis of monoclonal antibody to CNAP1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CNAP1 Antibody (4C12) [H00009918-M01]

Immunohistochemistry-Paraffin: CNAP1 Antibody (4C12) [H00009918-M01]

Immunohistochemistry-Paraffin: CNAP1 Antibody (4C12) [H00009918-M01] - Analysis of monoclonal antibody to CNAP1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
Western Blot: CNAP1 Antibody (4C12) [H00009918-M01]

Western Blot: CNAP1 Antibody (4C12) [H00009918-M01]

Western Blot: CNAP1 Antibody (4C12) [H00009918-M01] - Analysis of CNAP1 expression in transfected 293T cell line by CNAP1 monoclonal antibody (M01), clone 4C12.Lane 1: CNAP1 transfected lysate(157 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: CNAP1 Antibody (4C12) [H00009918-M01] - Detection limit for recombinant GST tagged CNAP1 is approximately 0.1ng/ml as a capture antibody.

Applications for CNAP1 Antibody (4C12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein, transfected lysate and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CNAP1

CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).

Alternate Names

CAPD2, CAP-D2, Chromosome condensation-related SMC-associated protein 1, Chromosome-associated protein D2, CNAP1XCAP-D2 homolog, hCAP-D2condensin complex subunit 1, KIAA0159chromosome condensation related SMC associated protein 1, Non-SMC condensin I complex subunit D2, non-SMC condensin I complex, subunit D2

Entrez Gene IDs

9918 (Human)

Gene Symbol

NCAPD2

Additional CNAP1 Products

Product Documents for CNAP1 Antibody (4C12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CNAP1 Antibody (4C12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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