![Western Blot: CNOT4 Antibody [NBP2-24527]](https://resources.rndsystems.com/images/products/CNOT4-Antibody-Western-Blot-NBP2-24527-img0001.jpg "Western Blot: CNOT4 Antibody [NBP2-24527]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Bovine, Chicken, Xenopus
Predicted:
Canine (100%), Chimpanzee (100%), Opossum (93%), Primate (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
A portion of amino acids 50-100 of human CNOT4 was used as the immunogen for the antibody.
Reactivity Notes
The amino acid sequence used as immunogen for the antibody is 86% homologous in Chicken.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CNOT4 Antibody
Western Blot: CNOT4 Antibody [NBP2-24527]
Western Blot: CNOT4 Antibody [NBP2-24527] - analysis of CNOT4 in human skeletal muscle lysate in the 1) absence and 2) presence of immunizing peptide using CNOT4 antibody at 0.05 ug/ml.![Immunohistochemistry-Paraffin: CNOT4 Antibody [NBP2-24527]](https://resources.rndsystems.com/images/products/CNOT4-Antibody-Immunohistochemistry-Paraffin-NBP2-24527-img0002.jpg "Immunohistochemistry-Paraffin: CNOT4 Antibody [NBP2-24527]")
Immunohistochemistry-Paraffin: CNOT4 Antibody [NBP2-24527]
Immunohistochemistry-Paraffin: CNOT4 Antibody [NBP2-24527] - Formalin-fixed paraffin-embedded human prostate tissue stained with CNOT4 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.Applications for CNOT4 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
10 ug/ml
Western Blot
0.05-0.1 ug/ml
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: CNOT4
Alternate Names
CCR4-associated factor 4, CCR4-NOT transcription complex, subunit 4, E3 ubiquitin-protein ligase CNOT4, EC 6.3.2.-, NOT4yeast) homolog
Gene Symbol
CNOT4
Additional CNOT4 Products
Product Documents for CNOT4 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CNOT4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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