Coagulation Factor II/Thrombin Antibody (1B7) - Azide and BSA Free

Novus Biologicals | Catalog # H00002147-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunoprecipitation, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 1B7

Format

Azide and BSA Free
View all Coagulation Factor II/Thrombin Primary Antibodies »

Product Specifications

Immunogen

F2 (AAH51332, 263 a.a. ~ 362 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RNPDGDEEGAWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDG

Localization

Secreted

Specificity

F2 - coagulation factor II (thrombin)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Coagulation Factor II/Thrombin Antibody (1B7) - Azide and BSA Free

Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01]

Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01]

Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody (M01), clone 1B7.Lane 1: F2 transfected lysate(70 KDa).Lane 2: Non-transfected lysate.
Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01]

Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01]

Western Blot: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi ( Cat # H00002147-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody (M01), clone 1B7 (Cat # H00002147-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Proximity Ligation Assay: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01]

Proximity Ligation Assay: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01]

Proximity Ligation Assay: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of protein-protein interactions between FGA and F2. HeLa cells were stained with anti-FGA rabbit purified polyclonal 1:1200 and anti-F2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunoprecipitation: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01]

Immunoprecipitation: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01]

Immunoprecipitation: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Analysis of F2 transfected lysate using anti-F2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with F2 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: Coagulation Factor II/Thrombin Antibody (1B7) [H00002147-M01] - Detection limit for recombinant GST tagged F2 is approximately 1ng/ml as a capture antibody.

Applications for Coagulation Factor II/Thrombin Antibody (1B7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Coagulation Factor II/Thrombin

Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia.

Alternate Names

F2, Thrombin

Entrez Gene IDs

2147 (Human)

Gene Symbol

F2

OMIM

176930 (Human)

UniProt

AAH51332 (Human)

Additional Coagulation Factor II/Thrombin Products

Product Documents for Coagulation Factor II/Thrombin Antibody (1B7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Coagulation Factor II/Thrombin Antibody (1B7) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Coagulation Factor II/Thrombin Antibody (1B7) - Azide and BSA Free

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    • A: We currently have 1 neutralizing Thrombin antibody (NBP1-95029), however it is an anti-human antibody and has only been validated in human samples. If you would like to test this antibody in mouse samples, you may be interested in participating in our Innovators Reward Program.
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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail