Cofilin Antibody (PSH0-34)

Western Blot: Cofilin Antibody (PSH0-34) [NBP3-32205]

Western Blot: Cofilin Antibody (PSH0-34) [NBP3-32205] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32205) at 1/1,000 dilution.

Lane 1: Hela cell lysate (30 ug/Lane)
Lane 2: HEK-293 cell lysate (30 ug/Lane)
Lane 3: MCF-7 cell lysate (30 ug/Lane)
Lane 4: MDA-MB-468 cell lysate (24 ug/Lane)
Lane 5: K-562 cell lysate (16 ug/Lane)
Lane 6: SH-SY5Y cell lysate (22 ug/Lane)
Lane 7: HUVEC cell lysate (30 ug/Lane)
Lane 8: Jurkat cell lysate (30 ug/Lane)
Lane 9: COS-1 cell lysate (30 ug/Lane)
Lane 10: VERO cell lysate (30 ug/Lane)
Lane 11: NIH/3T3 cell lysate (22 ug/Lane)
Lane 12: PC-12 cell lysate (19 ug/Lane)
Lane 13: Neuro-2a cell lysate (19 ug/Lane)

Predicted band size: 19 kDa
Observed band size: 19 kDa

Exposure time: 3 minutes;

15% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32205) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: Cofilin Antibody (PSH0-34) [NBP3-32205]

Flow Cytometry: Cofilin Antibody (PSH0-34) [NBP3-32205] - Flow cytometric analysis of Hela cells labeling Cofilin.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32205, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).