Coilin Antibody (7Z4K1)

Novus Biologicals | Catalog # NBP3-16196

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7Z4K1 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Coilin (P38432). MAASETVRLRLQFDYPPPATPHCTAFWLLVDLNRCRVVTDLISLIRQRFGFSSGAFLGLYLEGGLLPPAESARLVRDNDCLRVKLEERGVAENSVVISNG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Coilin Antibody (7Z4K1)

Western Blot: Coilin Antibody (7Z4K1) [NBP3-16196]

Western Blot: Coilin Antibody (7Z4K1) [NBP3-16196]

Western Blot: Coilin Antibody (7Z4K1) [NBP3-16196] - Western blot analysis of extracts of various cell lines, using Coilin Rabbit mAb (NBP3-16196) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for Coilin Antibody (7Z4K1)

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Coilin

Coilin is encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.

Alternate Names

CLN80p80-coilin, coilin, coilin p80, p80

Gene Symbol

COIL

Additional Coilin Products

Product Documents for Coilin Antibody (7Z4K1)

Certificate of Analysis

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Product Specific Notices for Coilin Antibody (7Z4K1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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