COL1A2 Antibody (HL2049) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25415
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL2049 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant fragment of human COL1A2
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
165 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for COL1A2 Antibody (HL2049) - Azide and BSA Free
Western Blot: COL1A2 Antibody (HL2049) - Azide and BSA Free [NBP3-25415] -
Western Blot: COL1A2 Antibody (HL2049) - Azide and BSA Free [NBP3-25415] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2049] (NBP3-25415) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Western Blot: COL1A2 Antibody (HL2049) - Azide and BSA Free [NBP3-25415] -
Western Blot: COL1A2 Antibody (HL2049) - Azide and BSA Free [NBP3-25415] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2049] (NBP3-25415) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Western Blot: COL1A2 Antibody (HL2049) - Azide and BSA Free [NBP3-25415] -
Western Blot: COL1A2 Antibody (HL2049) - Azide and BSA Free [NBP3-25415] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2049] (NBP3-25415) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.Applications for COL1A2 Antibody (HL2049) - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: COL1A2
Alternate Names
alpha 2(I)-collagen, Alpha-2 type I collagen, collagen alpha-2(I) chain, collagen I, alpha-2 polypeptide, collagen of skin, tendon and bone, alpha-2 chain, collagen, type I, alpha 2, OI4, osteogenesis imperfecta type IV, type I procollagen
Gene Symbol
COL1A2
Additional COL1A2 Products
Product Documents for COL1A2 Antibody (HL2049) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for COL1A2 Antibody (HL2049) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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