Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all COL4A3BP Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 300 and 350 of human CERTL(GPBP) using the numbering given in entry NP_005704.1 (GeneID 10087).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COL4A3BP Antibody - BSA Free

Western Blot: COL4A3BP Antibody [NB100-2114]

Western Blot: COL4A3BP Antibody [NB100-2114]

Western Blot: COL4A3BP Antibody [NB100-2114] - Detection of Human CERT(GPBP) on HeLa whole cell lysate using NB100-2114. CERT(GPBP) was also immunoprecipitated using rabbit anti-CERT(GPBP) antibody NB100-2113.

Applications for COL4A3BP Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-4 ug/mg of lysate

Western Blot

1:2500-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: COL4A3BP

CERT (GPBP) is a kinase that phosphorylates the alpha 3 chain of type IV collagen, also known as the Goodpasture antigen, on serine and threonine residues. One isoform of CERT has been shown to mediate intracellular trafficking of ceramides from the endoplasmic reticulum to the Golgi apparatus in a non-vesicular manner. Alternate designations for CERT include Goodpasture antigen-binding protein, GPBP, collagen type IV alpha-3-binding protein, StAR-related lipid transfer protein 11, StARD11, START domain-containing protein 11, COL4A3BP, STARD11, CERTL, and FLJ20597.

Alternate Names

Ceramide transfer protein, CERTFLJ20597, CERTL, collagen type IV alpha-3-binding protein, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein, Goodpasture antigen-binding protein, GPBPSTARD11ceramide transporter, hCERT, lipid-transfer protein CERTL, StARD11, StAR-related lipid transfer (START) domain containing 11, StAR-related lipid transfer protein 11, START domain containing 11, START domain-containing protein 11

Entrez Gene IDs

10087 (Human)

Gene Symbol

CERT1

UniProt

Q9Y5P4 (Human)

Additional COL4A3BP Products

Product Documents for COL4A3BP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for COL4A3BP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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