Collagens comprise a large family of insoluble extracellular glycoproteins that are essential components of connective tissues such as tendons, ligaments, cartilage, bone and skin. The mature polypeptides are secreted as coiled, left-handed helices that subsequently assemble into rope-like collagen fibers. Collagen I is a fibril-forming collagen that requires N- and C- terminal processing. Collagen IV is a network forming collagen whose C-terminus forms dimers and N-terminus forms tetramers.
Collagen IV Antibody (rCOL4/4742)
Novus Biologicals | Catalog # NBP3-07596
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG2a Kappa Clone # rCOL4/4742
Loading...
Product Specifications
Immunogen
Purified human placental extract
Localization
Secreted. Extracellular matrix.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08487)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Collagen IV Antibody (rCOL4/4742)
![Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596]](https://resources.rndsystems.com/images/products/Collagen-IV-Antibody-rCOL4-4742-Immunohistochemistry-Paraffin-NBP3-07596-img0002.jpg "Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596]")
Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596]
Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596] - Formalin-fixed, paraffin-embedded human kidney stained with Collagen IV Recombinant Mouse Monoclonal Antibody (rCOL4/4742).![Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596]](https://resources.rndsystems.com/images/products/Collagen-IV-Antibody-rCOL4-4742-Immunohistochemistry-Paraffin-NBP3-07596-img0001.jpg "Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596]")
Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596]
Immunohistochemistry-Paraffin: Collagen IV Antibody (rCOL4/4742) [NBP3-07596] - Formalin-fixed, paraffin-embedded human kidney stained with Collagen IV Recombinant Mouse Monoclonal Antibody (rCOL4/4742)Applications for Collagen IV Antibody (rCOL4/4742)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Collagen IV
Alternate Names
BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR
Gene Symbol
COL4A1
UniProt
Additional Collagen IV Products
Product Documents for Collagen IV Antibody (rCOL4/4742)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Collagen IV Antibody (rCOL4/4742)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for Collagen IV Antibody (rCOL4/4742)
There are currently no reviews for this product. Be the first to review Collagen IV Antibody (rCOL4/4742) and earn rewards!
Have you used Collagen IV Antibody (rCOL4/4742)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
