CometAssay Single Cell Gel Electrophoresis Assay-Silver
R&D Systems | Catalog # 4251-050-K
Key Product Details
Features
Key Benefits
- Allows visualization by standard light microscopy
- Permanent staining for archiving samples
- Optimized for CometSlide staining
Product Summary for CometAssay Single Cell Gel Electrophoresis Assay-Silver
NOTE: Once reconstituted 2X Staining Reagent #4 is stable for 3 months at 4°C.
Kit Contents for CometAssay Single Cell Gel Electrophoresis Assay-Silver
- CometAssay Lysis Solution
- CometAssay LMAgarose
- CometSlides (2 Well, 25 Slides)
- 200 mM EDTA, pH 10
- 20X Staining Reagent #1
- 20X Staining Reagent #2
- 20X Staining Reagent #3
- 2X Staining Reagent #4
- 10X Fixation Additive< /br>Note: The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging.
Formulation, Preparation, and Storage
Shipping
Storage
Product Documents for CometAssay Single Cell Gel Electrophoresis Assay-Silver
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Citations for CometAssay Single Cell Gel Electrophoresis Assay-Silver
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FAQs for CometAssay Single Cell Gel Electrophoresis Assay-Silver
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Q: How long can CometSlides™ be stored after electrophoresis?
A: Prior to staining, slides immersed in 70% ethanol for 5 minutes and then dried can be stored at room temperature for 1 year prior to staining. Permanent records can be created after staining for visualization by standard light microscopy using the CometAssay® Silver Staining Kit (Catalog # 4251-050-K).
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Q: What can cause observation of artefacts during imaging of Comet data?
A: For optimal imaging, all cells should be in the same plane and this is achieved by completely drying the agarose. Artefacts may be introduced if the source cells used for analyis has any debris. The debris should be allowed to settle before pipetting cells. The LMagarose can also be the cause of artefacts. To prevent debris from accumulating in the agar, melt it at 80-90 °C so it is runny, spin it at low speed and aliquot into sterile tubes in a hood. Use each aliquot for a single assay.
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Q: How long can CometSlides™ be stored after electrophoresis?
A: Prior to staining, slides immersed in 70% ethanol for 5 minutes and then dried can be stored at room temperature for 1 year prior to staining. Permanent records can be created after staining for visualization by standard light microscopy using the CometAssay® Silver Staining Kit (Catalog # 4251-050-K).
-
Q: What can cause observation of artefacts during imaging of Comet data?
A: For optimal imaging, all cells should be in the same plane and this is achieved by completely drying the agarose. Artefacts may be introduced if the source cells used for analyis has any debris. The debris should be allowed to settle before pipetting cells. The LMagarose can also be the cause of artefacts. To prevent debris from accumulating in the agar, melt it at 80-90 °C so it is runny, spin it at low speed and aliquot into sterile tubes in a hood. Use each aliquot for a single assay.