CometAssay Single Cell Gel Electrophoresis Assay

Catalog # Availability Size / Price Qty
4250-050-K
Product Details
FAQs
Reviews

CometAssay Single Cell Gel Electrophoresis Assay Summary

Includes twenty-five specially treated CometSlides (2 Well) and sufficient reagents for 50 test samples.

Key Benefits

• Specially treated slides to promote agarose adherence
• Ready to use low melt agarose (LMAgarose) in convenient size
• Lysis Solution suitable for either alkaline or neutral comet assay
• Optimized electrophoresis conditions

Product Specifications

  • Applicable for the analysis of either single- or double-strand DNA breaks
  • Study cellular responses to DNA damage
  • Screen for inhibitors of DNA repair
  • Test chemicals for toxicity
  • Screen for environmental mutagens

What is the CometAssay?

The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA “comet” tail shape and migration pattern allows for assessment of DNA damage. The general Comet assay protocol includes first immobilizing cells in a bed of low melting point agarose, gently lysing the cells, treating cells with neutral or alkali solutions to unwind and denature the DNA as well as hydrolyze sites of damage. DNA is then subjected to electrophoresis and stained with a fluorescent DNA intercalating dye.

Kit Contents

• CometAssay Lysis Solution
• CometAssay LMAgarose
• CometSlides (2 Well, 25 Slides)
• 200 mM EDTA, pH 10

Kit Contents

  • CometAssay Lysis Solution
  • CometAssay LMAgarose
  • CometSlides (2 Well, 25 Slides)
  • 200 mM EDTA, pH 10

Specifications

Shipping Conditions
The product is shipped ambient or with polar packs. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage
Store products immediately at the temperature recommended on the product labels.
Species
N/A

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Reagents Provided

Other Reagents Provided

FAQs

No product specific FAQs exist for this product, however you may

View all FAQs

Reviews for CometAssay Single Cell Gel Electrophoresis Assay

There are currently no reviews for this product. Be the first to review CometAssay Single Cell Gel Electrophoresis Assay and earn rewards!

Have you used CometAssay Single Cell Gel Electrophoresis Assay?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review