CometSlide

Catalog # Availability Size / Price Qty
4250-004-03
4250-050-03
4250-200-03
Product Details
Citations (33)
FAQs
Reviews (1)

CometSlide Summary

A 2-well slide that sample-ready, pretreated, and optimized for use in the CometAssay System.

Why Use CometSlides for the CometAssay?

CometSlide is a component of the CometAssay Kit (R&D Systems®, Catalog # 4250-050-K) which is used for the rapid analysis of DNA fragmentation associated with DNA damage. CometSlide is a 2-well slide that is specially treated to promote agarose adherence and easy application of cells directly to each well. This slide provides a convenient, simple, and reliable format for sample preparation, processing, analysis, and storage for the CometAssay.

The CometAssay, or single cell gel electrophoresis assay, permits the quantification of DNA damage in individual cells based on the migration of DNA structures in an electric field. Evaluation of the DNA “Comet” tail shape and migration pattern allows for assessment of DNA damage, expressed as tail length, percent DNA in the tail and tail moment.

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended on the label.
Storage
Store the unopened product at room temperature. Do not use past expiration date.
Species
N/A

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

Citations for CometSlide

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Barrier-to-autointegration factor 1 (Banf1) regulates poly [ADP-ribose] polymerase 1 (PARP1) activity following oxidative DNA damage
    Authors: E Bolderson, JT Burgess, J Li, NS Gandhi, D Boucher, LV Croft, S Beard, JJ Plowman, A Suraweera, MN Adams, A Naqi, SD Zhang, DA Sinclair, KJ O'Byrne, DJ Richard
    Nat Commun, 2019;10(1):5501.  2019
  2. Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression
    Authors: NP Montaldo, DL Bordin, A Brambilla, M Rösinger, SL Fordyce Ma, KØ Bjørås, S Bradamante, PA Aas, A Furrer, LC Olsen, N Kunath, M Otterlei, P Sætrom, M Bjørås, LD Samson, B van Loon
    Nat Commun, 2019;10(1):5460.  2019
  3. Inducing and exploiting vulnerabilities for the treatment of liver cancer
    Authors: C Wang, S Vegna, H Jin, B Benedict, C Lieftink, C Ramirez, RL de Oliveir, B Morris, J Gadiot, W Wang, A du Chatini, L Wang, D Gao, B Evers, G Jin, Z Xue, A Schepers, F Jochems, AM Sanchez, S Mainardi, H Te Riele, RL Beijersber, W Qin, L Akkari, R Bernards
    Nature, 2019;0(0):.  2019
  4. Recovery from repeated stressors: Physiology and behavior are affected on different timescales in house sparrows
    Authors: BMG Gormally, R Estrada, H Yin, LM Romero
    Gen. Comp. Endocrinol., 2019;282(0):113225.  2019
  5. Abnormal leaf development of rpt5a mutant under zinc deficiency reveals important role of DNA damage alleviation for normal leaf development
    Authors: N Sotta, T Sakamoto, S Matsunaga, T Fujiwara
    Sci Rep, 2019;9(1):9369.  2019
  6. Cell line-specific efficacy of thermoradiotherapy in human and canine cancer cells in vitro
    Authors: KJ Nytko, P Thumser-He, MS Weyland, S Scheidegge, CR Bley
    PLoS ONE, 2019;14(5):e0216744.  2019
  7. Phytoremediation efficacy assessment of polycyclic aromatic hydrocarbons contaminated soils using garden pea (Pisum sativum) and earthworms (Eisenia fetida)
    Authors: AK Sivaram, P Logeshwara, R Lockington, R Naidu, M Megharaj
    Chemosphere, 2019;229(0):227-235.  2019
  8. O-GlcNAcylation enhances double strand break repair, promotes cancer cell proliferation and prevents therapy-induced senescence in irradiated tumors
    Authors: EV Efimova, OK Appelbe, N Ricco, SS Lee, Y Liu, DJ Wolfgeher, TN Collins, AC Flor, A Ramamurthy, S Warrington, VP Bindokas, SJ Kron
    Mol. Cancer Res., 2019;0(0):.  2019
  9. PRRX1 isoforms cooperate with FOXM1 to regulate the DNA damage response in pancreatic cancer cells
    Authors: B Marchand, JR Pitarresi, M Reichert, K Suzuki, D Laczkó, AK Rustgi
    Oncogene, 2019;0(0):.  2019
  10. Spatial and temporal variation of three biomarkers in Mytilus edulis
    Authors: S Strubbia, BP Lyons, RJ Lee
    Mar. Pollut. Bull., 2019;138(0):322-327.  2019
  11. CtIP-BRCA1 complex and MRE11 maintain replication forks in the presence of chain terminating nucleoside analogs
    Authors: M Mohiuddin, MM Rahman, JE Sale, CE Pearson
    Nucleic Acids Res., 2019;0(0):.  2019
  12. STAT5A/B BLOCKADE SENSITIZES PROSTATE CANCER TO RADIATION THROUGH INHIBITION OF RAD51 AND DNA REPAIR
    Authors: C Maranto, V Udhane, DT Hoang, L Gu, V Alexeev, KM Malas, K Cardenas, JR Brody, U Rodeck, C Bergom, KA Iczkowski, K Jacobsohn, WA See, SM Schmitt, MT Nevalainen
    Clin. Cancer Res., 2018;0(0):.  2018
  13. Targeting the lysosome by an aminomethylated Riccardin D triggers DNA damage through cathepsin B-mediated degradation of BRCA1
    Authors: Y Wang, H Niu, Z Hu, M Zhu, L Wang, L Han, L Qian, K Tian, H Yuan, H Lou
    J. Cell. Mol. Med., 2018;0(0):.  2018
  14. Marginal Zinc Deficiency and Environmentally Relevant Concentrations of Arsenic Elicit Combined Effects on the Gut Microbiome
    Authors: CA Gaulke, J Rolshoven, CP Wong, LG Hudson, E Ho, TJ Sharpton
    mSphere, 2018;3(6):.  2018
  15. Sequential combination therapy of ovarian cancer with degradable N-(2-hydroxypropyl)methacrylamide copolymer paclitaxel and gemcitabine conjugates.
    Authors: Zhang R, Yang J, Sima M, Zhou Y, Kopecek J
    Proc Natl Acad Sci U S A, 0;111(33):12181-6.  0
  16. B1, a novel HDAC inhibitor, induces apoptosis through the regulation of STAT3 and NF-kappaB.
    Authors: Cheng M, Wong Y, Chang C, Yang C, Chen S, Yuan C, Kuo H, Yang C, Chiu H
    Int J Mol Med, 0;39(5):1137-1148.  0
  17. Oncolytic virus-mediated manipulation of DNA damage responses: synergy with chemotherapy in killing glioblastoma stem cells.
    Authors: Kanai R, Rabkin S, Yip S, Sgubin D, Zaupa C, Hirose Y, Louis D, Wakimoto H, Martuza R
    J Natl Cancer Inst, 0;104(1):42-55.  0
  18. Validation of freezing tissues and cells for analysis of DNA strand break levels by comet assay.
    Authors: Jackson P, Pedersen L, Kyjovska Z, Jacobsen N, Saber A, Hougaard K, Vogel U, Wallin H
    Mutagenesis, 0;28(6):699-707.  0
  19. Effects of chronic exposure to an environmentally relevant mixture of brominated flame retardants on the reproductive and thyroid system in adult male rats.
    Authors: Ernest S, Wade M, Lalancette C, Ma Y, Berger R, Robaire B, Hales B
    Toxicol Sci, 0;127(2):496-507.  0
  20. Intracellular deoxyribonucleotide pool imbalance and DNA damage in cells treated with hydroxyurea, an inhibitor of ribonucleotide reductase.
    Authors: Osterman Golkar S, Czene S, Gokarakonda A, Haghdoost S
    Mutagenesis, 0;28(6):653-60.  0
  21. CK2 inhibitor CX-4945 suppresses DNA repair response triggered by DNA-targeted anticancer drugs and augments efficacy: mechanistic rationale for drug combination therapy.
    Authors: Siddiqui-Jain A, Bliesath J, Macalino D, Omori M, Huser N, Streiner N, Ho C, Anderes K, Proffitt C, O'Brien S, Lim J, Von Hoff D, Ryckman D, Rice W, Drygin D
    Mol Cancer Ther, 0;11(4):994-1005.  0
  22. The S229L colon tumor-associated variant of DNA polymerase beta induces cellular transformation as a result of decreased polymerization efficiency.
    Authors: Nemec A, Murphy D, Donigan K, Sweasy J
    J Biol Chem, 0;289(20):13708-16.  0
  23. Nongenotoxic apoptosis inducers do not produce misleading positive results in the TK6 cell-based GADD45a-GFP genotoxicity assay.
    Authors: Topham C, Billinton N, Walmsley R
    Toxicol Sci, 0;128(1):79-91.  0
  24. Y265C DNA polymerase beta knockin mice survive past birth and accumulate base excision repair intermediate substrates.
    Authors: Senejani A, Dalal S, Liu Y, Nottoli T, McGrath J, Clairmont C, Sweasy J
    Proc Natl Acad Sci U S A, 0;109(17):6632-7.  0
  25. Silencing of human DNA polymerase lambda causes replication stress and is synthetically lethal with an impaired S phase checkpoint.
    Authors: Zucca E, Bertoletti F, Wimmer U, Ferrari E, Mazzini G, Khoronenkova S, Grosse N, van Loon B, Dianov G, Hubscher U, Maga G
    Nucleic Acids Res, 0;41(1):229-41.  0
  26. Toxicological profile of small airway epithelial cells exposed to gold nanoparticles.
    Authors: Ng C, Li J, Gurung R, Hande M, Ong C, Bay B, Yung L
    Exp Biol Med (Maywood), 0;238(12):1355-61.  0
  27. FASN regulates cellular response to genotoxic treatments by increasing PARP-1 expression and DNA repair activity via NF-κB and SP1.
    Authors: Wu X, Dong Z, Wang C, Barlow L, Fako V, Serrano M, Zou Y, Liu J, Zhang J
    Proc Natl Acad Sci U S A, 0;0(0):.  0
  28. Sexual signals reflect telomere dynamics in a wild bird.
    Authors: Taff C, Freeman-Gallant C
    Ecol Evol, 0;7(10):3436-3442.  0
  29. Mitochondrial reactive oxygen species are scavenged by Cockayne syndrome B protein in human fibroblasts without nuclear DNA damage.
    Authors: Cleaver J, Brennan-Minnella A, Swanson R, Fong K, Chen J, Chou K, Chen Y, Revet I, Bezrookove V
    Proc Natl Acad Sci U S A, 0;111(37):13487-92.  0
  30. Caspase-9 is required for normal hematopoietic development and protection from alkylator-induced DNA damage in mice.
    Authors: Lu E, McLellan M, Ding L, Fulton R, Mardis E, Wilson R, Miller C, Westervelt P, DiPersio J, Link D, Walter M, Ley T, Graubert T
    Blood, 0;124(26):3887-95.  0
  31. The GH/IGF-1 axis in a critical period early in life determines cellular DNA repair capacity by altering transcriptional regulation of DNA repair-related genes: implications for the developmental origins of cancer.
    Authors: Podlutsky A, Valcarcel-Ares M, Yancey K, Podlutskaya V, Nagykaldi E, Gautam T, Miller R, Sonntag W, Csiszar A, Ungvari Z
    Geroscience, 0;39(2):147-160.  0
  32. Synthetic beta-nitrostyrene derivative CYT-Rx20 as inhibitor of oral cancer cell proliferation and tumor growth through glutathione suppression and reactive oxygen species induction.
    Authors: Wang Y, Chen Y, Hsu Y, Chiu W, Tsai C, Hu S, Hsieh P, Yuan S
    Head Neck, 0;39(6):1055-1064.  0
  33. ATM regulates NF-kappaB-dependent immediate-early genes via RelA Ser 276 phosphorylation coupled to CDK9 promoter recruitment.
    Authors: Fang L, Choudhary S, Zhao Y, Edeh C, Yang C, Boldogh I, Brasier A
    Nucleic Acids Res, 0;42(13):8416-32.  0

FAQs

  1. Can 2-Well CometSlides™ be used instead of the 3-Well FLARE™ Slide?

    • The well size for the CometSlide™ 2- well and the FLARE™ Slide 3-well are identical. The slides are interchangeable.

  2. Is it necessary to use a coverslip with CometSlides™?

    • FLARE™ and CometSlides™ were designed to be used without coverslips because removal of the coverslip could cause detachment of the agarose and cause damage to the samples.

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CometSlide
By Anonymous on 05/16/2018
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