COMP/Thrombospondin-5 Antibody [mFluor Violet 500 SE]
Novus Biologicals | Catalog # AF3134MFV500
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Neutralization
Label
mFluor Violet 500 SE (Excitation = 410 nm, Emission = 501 nm)
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human COMP/Thrombospondin-5
Gln21-Ala757 (Ala256Arg)
Accession # P49747
Gln21-Ala757 (Ala256Arg)
Accession # P49747
Specificity
Detects human COMP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse COMP is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for COMP/Thrombospondin-5 Antibody [mFluor Violet 500 SE]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: COMP/Thrombospondin-5
Long Name
Cartilage Oligomeric Matrix Protein
Alternate Names
EDM1, EPD1, MED, PSACH, Thrombospondin-5, Thrombospondin5
Gene Symbol
COMP
Additional COMP/Thrombospondin-5 Products
Product Documents for COMP/Thrombospondin-5 Antibody [mFluor Violet 500 SE]
Product Specific Notices for COMP/Thrombospondin-5 Antibody [mFluor Violet 500 SE]
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for COMP/Thrombospondin-5 Antibody [mFluor Violet 500 SE]
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Q: I have synovial fluid sample and I want to work on Western bloting can you support me,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?
A: We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374
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Associated Pathways
