|Detection of Human COMP/Thrombospondin‑5 by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Human COMP/Thrombospondin‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for COMP/Thrombospondin‑5 at approximately 90 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Cell Adhesion Mediated by COMP/Thrombospondin‑5 and Neutralization by Human COMP/Thrombospondin‑5 Antibody. Recombinant Human COMP/Thrombospondin‑5 (Catalog # 3134-CP), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human COMP/Thrombospondin‑5 (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human COMP/Thrombospondin‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134). The ND50 is typically|
Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). The human COMP cDNA encodes a 757 amino acid (aa) precursor that includes a 20 aa signal sequence followed by a non-collagenous
coiled‑coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain (5). Human COMP shares 86-93% aa sequence identity with rat, mouse, equine, bovine, and canine COMP. Within the TSP type-3 repeats and TSP C-terminal domain, human COMP shares 60%, 61%, 74%, and 80% aa sequence identity with human Thrombospondin-1, -2, -3, and -4, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).