Human COMP/Thrombospondin-5 Antibody

Catalog # Availability Size / Price Qty
AF3134
AF3134-SP
Human COMP/Thrombospondin-5 Antibody in Western Blot
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Citations (2)
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Human COMP/Thrombospondin-5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human COMP in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant human Thrombospondin-1, -2, -3 and -4 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human COMP/Thrombospondin-5
Gln21-Ala757 (Ala256Arg)
Accession # P49747
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below
Neutralization
Measured by its ability to neutralize COMP/Thrombospondin‑5-mediated adhesion of the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 1-4 µg/mL in the presence of 10 µg/mL Recombinant Human COMP/Thrombospondin‑5.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human COMP/Thrombospondin‑5 by Western Blot. View Larger

Detection of Human COMP/Thrombospondin‑5 by Western Blot. Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Human COMP/Thrombospondin‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for COMP/Thrombospondin‑5 at approximately 90 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Cell Adhesion Mediated by COMP/Thrombospondin‑5 and Neutralization by Human COMP/Thrombospondin‑5 Antibody. View Larger

Cell Adhesion Mediated by COMP/Thrombospondin‑5 and Neutralization by Human COMP/Thrombospondin‑5 Antibody. Recombinant Human COMP/Thrombospondin‑5 (Catalog # 3134-CP), immobilized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human COMP/Thrombospondin‑5 (10 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human COMP/Thrombo­spondin‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3134). The ND50 is typically
1-4 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COMP/Thrombospondin-5

Cartilage Oligomeric Matrix Protein (COMP), also known as Thrombospondin-5, is a 110 kDa multidomain calcium binding protein that associates with other extracellular matrix molecules. Thrombospondin-1 and -2 constitute subgroup A and form homotrimers, whereas Thrombospondin-3, -4, and COMP constitute subgroup B and form homopentamers (1-4). The human COMP cDNA encodes a 757 amino acid (aa) precursor that includes a 20 aa signal sequence followed by a non-collagenous
coiled‑coil domain, four EGF-like repeats, seven TSP type-3 repeats, and a globular TSP C-terminal domain (5). Human COMP shares 86-93% aa sequence identity with rat, mouse, equine, bovine, and canine COMP. Within the TSP type-3 repeats and TSP C-terminal domain, human COMP shares 60%, 61%, 74%, and 80% aa sequence identity with human Thrombospondin-1, -2, -3, and -4, respectively. The coiled coil domain mediates the association of COMP into disulfide-linked homopentamers with a central hub and peripheral globular domains connected by flexible strands (6, 7). An axial pore is formed by the coiled coil assembly and binds vitamin D3 which is involved in bone and cartilage metabolism (8). An RGD sequence in the third TSP type-3 repeat mediates chondrocyte attachment via Integrin alpha 5 beta 1, although when reduced and in the absence of calcium, attachment is mediated via Integrin alpha V beta 3 (9). COMP is upregulated in rheumatoid arthritis and osteoarthritis, hepatocellular carcinomas, chronic pancreatitis, and pancreatic carcinomas (10-12). Elevated circulating COMP levels are used as a biomarker for early onset of some skeletal disorders (10). Several mutations are associated with skeletal dysplasias, and the most common, a point mutation in the third TSP type-3 repeat, results in diminished calcium binding ability (13, 14).

References
  1. Adams, J.C. and J. Lawler (2004) Int J. Biochem. Cell Biol. 36:961.
  2. Posey, K.L. et al. (2004) Int. J. Biochem. Cell Biol. 36:1005.
  3. Adams, J.C. (2004) Int. J. Biochem. Cell Biol. 36:1102.
  4. Mann, H.H. et al. (2004) J. Biol. Chem. 279:25294.
  5. Newton, G. et al. (1994) Genomics, 24:435.
  6. DiCesare, P. et al. (1995) J. Orthopaedic Res. 13:422.
  7. Efimov, V.P. et al. (1994) FEBS Lett. 341:54.
  8. Ozbek, S. et al. (2002) EMBO J. 21:5960.
  9. Chen, F.H. et al. (2005) J. Biol. Chem. 280:32655.
  10. Wislowska, M. and B. Jablonska (2005) Clin. Rheumatol. 24:278.
  11. Xiao, Y. et al. (2004) J. Gastroenterol. Hepatol. 19:296.
  12. Liao, Q. et al. (2003) Scand. J. Gastroenterol. 38:207.
  13. Kennedy, J. et al. (2005) Eur. J. Hum. Genet. 13:547.
  14. Hou, J. et al. (2000) Cell Calcium 27:309.
Long Name
Cartilage Oligomeric Matrix Protein
Entrez Gene IDs
1311 (Human); 12845 (Mouse)
Alternate Names
cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5

Product Datasheets

Citations for Human COMP/Thrombospondin-5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Resolvin D1 prevents epithelial-mesenchymal transition and reduces the stemness features of hepatocellular carcinoma by inhibiting paracrine of cancer-associated fibroblast-derived COMP
    Authors: L Sun, Y Wang, L Wang, B Yao, T Chen, Q Li, Z Liu, R Liu, Y Niu, T Song, Q Liu, K Tu
    J. Exp. Clin. Cancer Res., 2019;38(1):170.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Cartilage oligomeric matrix protein is differentially expressed in human subcutaneous adipose tissue and regulates adipogenesis
    Authors: N Denton, KE Pinnick, F Karpe
    Mol Metab, 2018;16(0):172-179.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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