Complement Component C9 Antibody (C9-6-25-5)

Novus Biologicals | Catalog # NBP3-11209

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # C9-6-25-5
View all Complement Component C9 Primary Antibodies »

Product Specifications

Immunogen

Murine C9 peptide

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Description

Novus Biologicals Rat Complement Component C9 Antibody (C9-6-25-5) (NBP3-11209) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Applications for Complement Component C9 Antibody (C9-6-25-5)

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Complement Component C9

The monoclonal antibody C9-5-48 recognizes mouse complement component C9. The complement system is an ancient proinflammatory and microbial destruction system, that may be considered part of both the innate and adaptive immune systems. It consists of the classical, alternative, and lectin-binding pathways. Each pathway is triggered in a distinct manner, yet all deposit C3 fragments on a target and engage a common terminal sequence called TCC or the "membrane attack complex" (MAC). In contrast to the activation pathways, which require enzymatic cleavage for activation, the terminal pathway relies on conformational changes induced by binding of the different subunits. TCC is composed of a complex of four complement proteins (C5b, C6, C7, and C8) which bind to the outer surface of the target plasma membrane, and many copies of a fifth protein (C9) that hookup to one another, forming a ring in the membrane. The ring structure formed by C9 is a pore in the membrane that allows free diffusion of molecules in and out of the cell. If enough pores form, the cell is no longer able to survive. The membrane attack complex is initiated when the complement protein, C5 convertase, cleaves C5 into C5a and C5b. Binding of C6 facilitates binding of C7 which alters the conformation of the complex. After binding of C8, a variable number of C9 molecules associate with the C5b678 complex, together constituting TCC. The formation of TCC causes lysis of cells or can trigger a variety of cellular metabolic pathways resulting in the synthesis and release of inflammatory mediators.

Alternate Names

complement component 9, complement component C9

Gene Symbol

C9

Additional Complement Component C9 Products

Product Documents for Complement Component C9 Antibody (C9-6-25-5)

Certificate of Analysis

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Product Specific Notices for Complement Component C9 Antibody (C9-6-25-5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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