Connexin 30.3/GJB4 Antibody (1E3-1C12) - Azide and BSA Free

Novus Biologicals | Catalog # H00127534-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E3-1C12

Format

Azide and BSA Free
View all Connexin 30.3/GJB4 Primary Antibodies »

Product Specifications

Immunogen

GJB4 (AAH34709, 1 a.a. ~ 266 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MNWAFLQGLLSGVNKYSTVLSRIWLSVVFIFRVLVYVVAAEEVWDDEQKDFVCNTKQPGCPNVCYDEFFPVSHVRLWALQLILVTCPSLLVVMHVAYREERERKHHLKHGPNAPSLYDNLSKKRGGLWWTYLLSLIFKAAVDAGFLYIFHRLYKDYDMPRVVACSVEPCPHTVDCYISRPTEKKVFTYFMVTTAAICILLNLSEVFYLVGKRCMEIFGPRHRRPRCRECLPDTCPPYVLSQGGHPEDGNSVLMKAGSAPVDAGGYP

Specificity

GJB4 - gap junction protein, beta 4 (connexin 30.3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Connexin 30.3/GJB4 Antibody (1E3-1C12) - Azide and BSA Free

Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]

Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]

Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - GJB4 monoclonal antibody (M01), clone 1E3-1C12 Analysis of GJB4 expression in Hela.
Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]

Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]

Western Blot: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - Analysis of GJB4 expression in transfected 293T cell line by GJB4 monoclonal antibody (M01), clone 1E3-1C12.Lane 1: GJB4 transfected lysate(30 KDa).Lane 2: Non-transfected lysate.
ELISA: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]

ELISA: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01]

ELISA: Connexin 30.3/GJB4 Antibody (1E3-1C12) [H00127534-M01] - Detection limit for recombinant GST tagged GJB4 is approximately 3ng/ml as a capture antibody.

Applications for Connexin 30.3/GJB4 Antibody (1E3-1C12) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate, transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Connexin 30.3/GJB4

The Connexin 30.3 / GJB4 gene encodes a transmembrane connexin protein that is a component of gap junctions. Mutations in this gene havebeen associated with erythrokeratodermia variabilis, progressive symmetric erythrokeratoderma and hearing impairment.(provided by RefSeq)

Alternate Names

CX30.3, GJB4

Entrez Gene IDs

127534 (Human)

Gene Symbol

GJB4

OMIM

133 (Human)

UniProt

AAH34709 (Human)

Additional Connexin 30.3/GJB4 Products

Product Documents for Connexin 30.3/GJB4 Antibody (1E3-1C12) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Connexin 30.3/GJB4 Antibody (1E3-1C12) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Ion Channels and Regulators

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