Cortactin [p Tyr466] Antibody - BSA Free

Novus Biologicals | Catalog # NB100-81871

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cortactin Primary Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from Human Cortactin around the phosphorylation site of tyrosine 466 (P-V-YP-E-T).

Modification

p Tyr466

Specificity

Detects endogenous levels of Cortactin only when phosphorylated at tyrosine 466.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cortactin [p Tyr466] Antibody - BSA Free

Western Blot: Cortactin [p Tyr466] Antibody [NB100-81871]

Western Blot: Cortactin [p Tyr466] Antibody [NB100-81871]

Western Blot: Cortactin [p Tyr466] Antibody [NB100-81871] - Analysis of extracts from Jurkat cells untreated or treated with H2O2 using Cortactin(Phospho-Tyr466) antibody
Western Blot: Cortactin [p Tyr466] Antibody [NB100-81871]

Western Blot: Cortactin [p Tyr466] Antibody [NB100-81871]

Western Blot: Cortactin [p Tyr466] Antibody [NB100-81871] - Analysis of extracts from JK cells, treated with H2O2 or calf intestinal phosphatase (CIP), using Cortactin (Phospho-Tyr466) Antibody.

Applications for Cortactin [p Tyr466] Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cortactin

Cortactin is an 80/85 kDa cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino terminal tandem repeats of a unique 37 amino acid sequence, which associates with actin, and a Src homology 3 (SH3) domain at the carboxyl terminus. Between the repeat and the SH3 domain, there is an alpha helical structure followed by a proline rich region. Between the proline rich region and the SH3 domain, there are a series of Src phosphorylation sites (Tyr421, Tyr470, and Tyr486). In vitro, cortactin binds to and cross links F actin into meshworks.

Alternate Names

Amplaxin, CTTN, EMS1

Entrez Gene IDs

2017 (Human); 13043 (Mouse)

Gene Symbol

CTTN

UniProt

Q14247 (Human)

Additional Cortactin Products

Product Documents for Cortactin [p Tyr466] Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cortactin [p Tyr466] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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