COX-2 Antibody (2V7G8)

Novus Biologicals | Catalog # NBP3-16220

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2V7G8 expressed in HEK293
View all COX-2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 505-604 of human COX-2 (P35354). GETMVEVGAPFSLKGLMGNVICSPAYWKPSTFGGEVGFQIINTASIQSLICNNVKGCPFTSFSVPDPELIKTVTINASSSRSGLDDINPTVLLKERSTEL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

69 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COX-2 Antibody (2V7G8)

Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220]

Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220]

Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of extracts of various cell lines, using COX-2 antibody (NBP3-16220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
COX-2 Antibody (2V7G8) - BSA Free

Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -

Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Human colon carcinoma tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
COX-2 Antibody (2V7G8) - BSA Free

Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -

Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Human colon tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
COX-2 Antibody (2V7G8) - BSA Free

Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -

Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Aanalysis of COX2/PTGS2 in paraffin-embedded mouse intestin tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
COX-2 Antibody (2V7G8) - BSA Free

Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -

Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of COX2/PTGS2 in paraffin-embedded rat colon tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for COX-2 Antibody (2V7G8)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunohistochemistry

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Western Blot

1:1000 - 1:6000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COX-2

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Gene Symbol

PTGS2

Additional COX-2 Products

Product Documents for COX-2 Antibody (2V7G8)

Certificate of Analysis

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Product Specific Notices for COX-2 Antibody (2V7G8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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