Rat COX-2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-49894

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 uL)

Sensitivity

75 pg/mL

Assay Range

125 - 8000 pg/mL
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Rat

Specificity

This kit recognizes Rat COX-2 in samples. No significant cross-reactivity or interference between Rat COX-2 and analogues was observed

Description

Assay Length: 3 h 30 min

Precision

Intra-Assay Precision (Precision within an assay) CV% 10

Inter-Assay Precision (Precision between assays) CV% 10

Recovery for Rat COX-2 ELISA Kit (Colorimetric)

Recovery

80 - 120%

Linearity

Rat COX-2 ELISA Kit (Colorimetric)
Rat COX-2 ELISA Kit (Colorimetric)

Scientific Data Images for Rat COX-2 ELISA Kit (Colorimetric)

Rat COX-2 ELISA Kit (Colorimetric)

ELISA: Rat COX-2 ELISA Kit (Colorimetric) [NBP3-49894]

Standard Curve Reference.

Kit Contents for Rat COX-2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Certificate of Analysis
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Description
  • Reference Standard
  • Reference Standard & Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: COX-2

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Gene Symbol

PTGS2

Additional COX-2 Products

Product Documents for Rat COX-2 ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rat COX-2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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