CPR8 Antibody

Western Blot: CPR8 Antibody [NBP3-32116]

Western Blot: CPR8 Antibody [NBP3-32116] - Western blot analysis of CPR8 on different lysates with Rabbit anti-CPR8 antibody (NBP3-32116) at 1/1,000 dilution.

Lane 1: HCT116 cell lysate
Lane 2: A549 cell lysate
Lane 3: Hela cell lysate
Lane 4: THP-1 cell lysate
Lane 5: SW480 cell lysate
Lane 6: HEK-293 cell lysate

Lysates/proteins at 30 ug/Lane.

Predicted band size: 87 kDa
Observed band size: 105 kDa

Exposure time: 2 minutes;

8% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32116) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: CPR8 Antibody [NBP3-32116]

Flow Cytometry: CPR8 Antibody [NBP3-32116] - Flow cytometric analysis of SW480 cells labeling CPR8.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32116, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).