Creatine Kinase BB Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-00288

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Creatine Kinase BB Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Creatine Kinase BB (Accession#: P12277; Pro2-Lys381)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Creatine Kinase BB Antibody - Azide and BSA Free

Creatine Kinase BB Antibody - Azide and BSA Free

Immunoprecipitation: Creatine Kinase BB Antibody [NBP3-00288] -

Immunoprecipitation: Creatine Kinase BB Antibody [NBP3-00288] - CKB was immunoprecipitated using: Lane A:0.5 mg A431 Whole Cell Lysate Lane B:0.5 mg Hela Whole Cell Lysate 2 uL NBP3-00288 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00288, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 43 kDa Observed band size: 43 kDa
Creatine Kinase BB Antibody - Azide and BSA Free

Western Blot: Creatine Kinase BB Antibody [NBP3-00288] -

Western Blot: Creatine Kinase BB Antibody [NBP3-00288] - NBP3-00288 at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lane C: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:43 kDa Observed band size:43 kDa

Applications for Creatine Kinase BB Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Product Documents for Creatine Kinase BB Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Creatine Kinase BB Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

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FAQs

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