CXCL1, CXCL2 (MIP-2 alpha), and CXCL3 (MIP-2 beta) are members of the alpha (C-X-C) subfamily of chemokines and are now designated GRO alpha), GRO beta, and GRO gamma, respectively. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.
CXCL1/2/3/GRO Antibody (186413) [PerCP]
Novus Biologicals | Catalog # FAB1114C
Key Product Details
Species Reactivity
Cotton Rat
Applications
Western Blot, Neutralization
Label
PerCP (Excitation = 490 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 186413
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Product Specifications
Immunogen
E. coli-derived recombinant cotton rat GRO
Ala28-Lys100
Accession # AAL16934
Ala28-Lys100
Accession # AAL16934
Specificity
Detects cotton rat CXCL1/2/3/GRO in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 5-20% cross-reactivity with recombinant rat CXCL1 and recombinant human CXCL2 and no cross-reactivity with recombinant cotton
rat CXCL10, recombinant human CXCL1, 3, 4, 5, 6, 7, 8, 9, 10, 11,
12/SDF-1 alpha, rh12/SDF-1 beta, 13, 16, recombinant mouse CXCL1, 2, 4, 6, 7, 9, 10, 11,
12/SDF-1 alpha, 13, 15, 16, 17,
recombinant rat (rr) CXCL2, rrCXCL3/CINC-2 alpha, rr3/CINC-2 beta, or recombinant porcine CXCL8.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for CXCL1/2/3/GRO Antibody (186413) [PerCP]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: CXCL1/2/3/GRO
Alternate Names
FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1
Additional CXCL1/2/3/GRO Products
Product Documents for CXCL1/2/3/GRO Antibody (186413) [PerCP]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CXCL1/2/3/GRO Antibody (186413) [PerCP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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