CXCL1, CXCL2 (MIP-2 alpha), and CXCL3 (MIP-2 beta) are members of the alpha (C-X-C) subfamily of chemokines and are now designated GRO alpha), GRO beta, and GRO gamma, respectively. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators.
CXCL1/2/3/GRO Antibody (186413) [CoraFluor™ 1]
Novus Biologicals | Catalog # FAB1114CL1
![CXCL1/2/3/GRO Antibody (186413) [CoraFluor™ 1]](https://resources.rndsystems.com/images/products/fab1114cl1_mouse-cxcl1-2-3-gro-mab-186413-corafluor-1-291020251214200.png "Product Feature: CoraFluor Probes for TR-FRET")
Key Product Details
Species Reactivity
Cotton Rat
Applications
Western Blot, Neutralization
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # 186413
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Product Specifications
Immunogen
E. coli-derived recombinant cotton rat GRO
Ala28-Lys100
Accession # AAL16934
Ala28-Lys100
Accession # AAL16934
Specificity
Detects cotton rat CXCL1/2/3/GRO in direct ELISAs and Western blots. In direct ELISAs, this antibody shows 5-20% cross-reactivity with recombinant rat CXCL1 and recombinant human CXCL2 and no cross-reactivity with recombinant cotton
rat CXCL10, recombinant human CXCL1, 3, 4, 5, 6, 7, 8, 9, 10, 11,
12/SDF-1 alpha, rh12/SDF-1 beta, 13, 16, recombinant mouse CXCL1, 2, 4, 6, 7, 9, 10, 11,
12/SDF-1 alpha, 13, 15, 16, 17,
recombinant rat (rr) CXCL2, rrCXCL3/CINC-2 alpha, rr3/CINC-2 beta, or recombinant porcine CXCL8.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
CoraFluor(TM) 1, amine reactive
CoraFluor(TM) 1, thiol reactive
For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
Scientific Data Images for CXCL1/2/3/GRO Antibody (186413) [CoraFluor™ 1]
Product Feature: CoraFluor Probes for TR-FRET
CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.Applications for CXCL1/2/3/GRO Antibody (186413) [CoraFluor™ 1]
Application
Recommended Usage
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: CXCL1/2/3/GRO
Alternate Names
FSP, GRO1, GROa, MGSA, MGSA-a, NAP-3, SCYB1
Additional CXCL1/2/3/GRO Products
Product Documents for CXCL1/2/3/GRO Antibody (186413) [CoraFluor™ 1]
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CXCL1/2/3/GRO Antibody (186413) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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