CXCR6 Antibody (JE66-40)

Novus Biologicals | Catalog # NBP3-32235

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE66-40 expressed in HEK293
View all CXCR6 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CXCR6 aa 1-100. (Uniprot: O00574)

Localization

Cell membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCR6 Antibody (JE66-40)

CXCR6 Antibody (JE66-40)

Western Blot: CXCR6 Antibody (JE66-40) [NBP3-32235]

Western Blot: CXCR6 Antibody (JE66-40) [NBP3-32235] - Western blot analysis of CXCR6 on different lysates with Rabbit anti-CXCR6 antibody (NBP3-32235) at 1/1,000 dilution.

Lane 1: JAR cell lysate (10 ug/Lane)
Lane 2: SW480 cell lysate (20 ug/Lane)
Lane 3: Human placenta tissue lysate (30 ug/Lane)

Predicted band size: 39 kDa
Observed band size: 39 kDa

Exposure time: 5 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32235) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Applications for CXCR6 Antibody (JE66-40)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CXCR6

Coreceptor usage of primary human immunodeficiency virus type 1 (HIV-1) isolates varies according to biological phenotype. The chemokine receptors CCR5 and CXCR4 are the major coreceptors that, together with CD4, govern HIV-1 entry into cells. Some HIV-1, HIV-2, and SIV isolates of different genetic subtypes and biological phenotypes use other chemokine receptors, such as CXCR6, a T-lymphocyte-expressed seven-transmembrane domain receptor that mediates fusion, entry, and trafficking of tumor-infiltrating lymphocytes. CXCR6 has been identified in human blood (T-lymphocytes, activated peripheral blood lymphocytes, monocyte-derived dendritic cells, primitive cord blood cells), placental trophoblasts, T-lymphocytes and dendritic cells in the skin, lymphoid tissues, and in fetal brain astrocytes. ESTs have been isolated from human normal placenta, liver/spleen, and pregnant uterus libraries, and human cancer kidney and uterus libraries.

Alternate Names

Bonzo, CD186, CXCR6, STRL33

Gene Symbol

CXCR6

Additional CXCR6 Products

Product Documents for CXCR6 Antibody (JE66-40)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CXCR6 Antibody (JE66-40)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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