CYB5R2 Antibody (OTI4B9) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-71942

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI4B9

Format

Azide and BSA Free
View all CYB5R2 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CYB5R2 (NP_057313) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

31.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CYB5R2 Antibody (OTI4B9) - Azide and BSA Free

Western Blot: CYB5R2 Antibody (OTI4B9)Azide and BSA Free [NBP2-71942]

Western Blot: CYB5R2 Antibody (OTI4B9)Azide and BSA Free [NBP2-71942]

Western Blot: CYB5R2 Antibody (OTI4B9) - Azide and BSA Free [NBP2-71942] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CYB5R2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CYB5R2.
Flow Cytometry: CYB5R2 Antibody (OTI4B9) - Azide and BSA Free [NBP2-71942]

Flow Cytometry: CYB5R2 Antibody (OTI4B9) - Azide and BSA Free [NBP2-71942]

Flow Cytometry: CYB5R2 Antibody (OTI4B9) - Azide and BSA Free [NBP2-71942] - HEK293T cells transfected with either CYB5R2 Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CYB5R2 antibody, and then analyzed by flow cytometry.

Applications for CYB5R2 Antibody (OTI4B9) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CYB5R2

NADH-cytochrome b5 reductases are involved in desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction. Responsible for NADH-dependent lucigenin chemiluminescence in spermatozoa by reducing both lucigenin and 2-[4-iodophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H tetrazolium monosodium salt (WST-1)

Alternate Names

B5R.2, cytochrome b5 reductase 2

Gene Symbol

CYB5R2

Additional CYB5R2 Products

Product Documents for CYB5R2 Antibody (OTI4B9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CYB5R2 Antibody (OTI4B9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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