CYB5R3 Antibody

Novus Biologicals | Catalog # NBP1-51984

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CYB5R3 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence PNLERVGHPKERC corresponding to C-Terminus according to Uniprot Mouse NP_084063.1.

Epitope

PNLERVGHPKERC

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for CYB5R3 Antibody

Western Blot: CYB5R3 Antibody [NBP1-51984]

Western Blot: CYB5R3 Antibody [NBP1-51984]

Western Blot: CYB5R3 Antibody [NBP1-51984] - Analysis of CYB5R3 in Mouse Liver lysate (RIPA buffer, 35ug total protein per lane) using NBP1-51984 at 0.1ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Applications for CYB5R3 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 1 ug/ml
Application Notes
WB: Approx. 35 kDa band observed in mouse liver lysates as well as in mouse and rat brain lysates (calculated MW of 34.1 kDa band according to mouse NP_084063.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CYB5R3

Two forms of NADH-cytochrome b5 reductase are known, a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum, mitochondrial and other membranes) and a soluble form in erythrocytes. The former exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids, in cholesterol biosynthesis, and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains. The soluble form has only the catalytic domain. This gene encodes both forms of the enzyme which arise from tissue-specific alternative transcripts that differ in the first exon. Mutations in this gene cause methemoglobinemias.

Alternate Names

B5RNADH-cytochrome b5 reductase 3, Cytochrome b5 reductase, cytochrome b5 reductase 3, DIA1NADH-cytochrome b5 reductase 3 membrane-bound form, diaphorase (NADH) (cytochrome b-5 reductase), diaphorase-1, EC 1.6.2.2, NADH-cytochrome b5 reductase 3 soluble form

Entrez Gene IDs

109754 (Mouse); 25035 (Rat)

Gene Symbol

CYB5R3

UniProt

NP_084063.1 (Mouse)

Additional CYB5R3 Products

Product Documents for CYB5R3 Antibody

Certificate of Analysis

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Product Specific Notices for CYB5R3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CYB5R3 Antibody

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