Cyclin A1/Cyclin A2 Antibody (10O1C8)
Novus Biologicals | Catalog # NBP3-16140
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10O1C8 expressed in HEK293
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Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human Cyclin A1/Cyclin A2 (P20248). RGKLQLVGTAAMLLASKFEEIYPPEVAEFVYITDDTYTKKQVLRMEHLVLKVLTFDLAAPTVNQFLTQYFLHQQPANCKVESLAMFLGELSLIDADPYLKYLPSVIAGAAFHLALYTVTGQSWPESLIRKTGYTLESLKPCLMDLHQTYLKAPQHAQQSIREKYKNSKYHGVSLLNPPETLNL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Cyclin A1/Cyclin A2 Antibody (10O1C8)
Western Blot: Cyclin A1/Cyclin A2 Antibody (10O1C8) [NBP3-16140]
Western Blot: Cyclin A1/Cyclin A2 Antibody (10O1C8) [NBP3-16140] - Western blot analysis of extracts of various cell lines, using Cyclin A1/Cyclin A2 antibody (NBP3-16140) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.Immunoprecipitation: Cyclin A1/Cyclin A2 Antibody (10O1C8) [NBP3-16140]
Immunoprecipitation: Cyclin A1/Cyclin A2 Antibody (10O1C8) [NBP3-16140] - Immunoprecipitation analysis of 600ug extracts of Mouse thymus using 3ug Cyclin A1/Cyclin A2 antibody (NBP3-16140). Western blot was performed from the immunoprecipitate using Cyclin A1/Cyclin A2 antibody (NBP3-16140) at a dilition of 1:1000.Applications for Cyclin A1/Cyclin A2 Antibody (10O1C8)
Application
Recommended Usage
Immunoprecipitation
1:500 - 1:1000
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cyclin A1/Cyclin A2
Alternate Names
CCN1, CCNA, CT146, cyclin-A1, cyclin-A2, testicular tissue protein Li 34
Gene Symbol
CCNA1
Additional Cyclin A1/Cyclin A2 Products
Product Documents for Cyclin A1/Cyclin A2 Antibody (10O1C8)
Certificate of Analysis
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Product Specific Notices for Cyclin A1/Cyclin A2 Antibody (10O1C8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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