Cyclin H Antibody (1B8) - Azide and BSA Free

Novus Biologicals | Catalog # H00000902-M01

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1B8

Format

Azide and BSA Free
View all Cyclin H Primary Antibodies »

Product Specifications

Immunogen

CCNH (AAH05280, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYHNSSQKRHWTFSSEEQLARLRADANRKFRCKAVANGKVLPNDPVFLEPHEEMTLCKYYEKRLLEFCSVFKPAMPRSVVGTACMYFKRFYLNNSVMEYHPRIIMLTCAF

Specificity

CCNH - cyclin H

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cyclin H Antibody (1B8) - Azide and BSA Free

Western Blot: Cyclin H Antibody (1B8) [H00000902-M01]

Western Blot: Cyclin H Antibody (1B8) [H00000902-M01]

Western Blot: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of CCNH expression in transfected 293T cell line by CCNH monoclonal antibody (M01), clone 1B8.Lane 1: CCNH transfected lysate(37.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (1B8) [H00000902-M01]

Immunocytochemistry/ Immunofluorescence: Cyclin H Antibody (1B8) [H00000902-M01]

Immunocytochemistry/Immunofluorescence: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: Cyclin H Antibody (1B8) [H00000902-M01]

Immunohistochemistry-Paraffin: Cyclin H Antibody (1B8) [H00000902-M01]

Immunohistochemistry-Paraffin: Cyclin H Antibody (1B8) [H00000902-M01] - Analysis of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
Western Blot: Cyclin H Antibody (1B8) [H00000902-M01]

Western Blot: Cyclin H Antibody (1B8) [H00000902-M01]

Western Blot: Cyclin H Antibody (1B8) [H00000902-M01] - CCNH monoclonal antibody (M01), clone 1B8 Analysis of CCNH expression in HeLa.
Sandwich ELISA: Cyclin H Antibody (1B8) [H00000902-M01] - Detection limit for recombinant GST tagged CCNH is approximately 1ng/ml as a capture antibody.

Applications for Cyclin H Antibody (1B8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cyclin H

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.

Alternate Names

CAK, cyclin H, cyclin-dependent kinase-activating kinase complex subunit, cyclin-H, MO15-associated protein, p34CAK complex subunit, p37CDK-activating kinase complex subunit

Entrez Gene IDs

902 (Human)

Gene Symbol

CCNH

OMIM

601953 (Human)

UniProt

AAH05280 (Human)

Additional Cyclin H Products

Product Documents for Cyclin H Antibody (1B8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cyclin H Antibody (1B8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cyclin H Antibody (1B8) - Azide and BSA Free

Customer Reviews for Cyclin H Antibody (1B8) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Cyclin H Antibody (1B8) - Azide and BSA Free and earn rewards!

Have you used Cyclin H Antibody (1B8) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies