Cyclophosphamide

Tocris Bioscience | Catalog # 4091

Alkylating agent; chemotherapeutic
Tocris Bioscience

Key Product Details

Description

Alkylating agent; chemotherapeutic
View all Apoptosis Inducer Compounds »

Product Description

Cyclophosphamide is a nitrogen mustard alkylating agent and prodrug. Phosphoramide mustard (active metabolite) forms DNA cross-links leading to cell death. Inhibits aldehyde dehydrogenase 1 (ALDH1) through its degradation product acrolein. Chemotherapeutic for the treatment of breast cancer; regulates Bax and Bcl-2 expression when administered with etoposide (Cat. No. 1226) in breast cancer cell lines.

Product Specifications for Cyclophosphamide

Molecular Weight

261.09

Formula

C7H15Cl2N2O2P

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

2-[bis(2-Chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 2-oxide

CAS Number

50-18-0

PubChem ID

2907

InChI Key

CMSMOCZEIVJLDB-UHFFFAOYSA-N

SMILES

O=P1(N(CCCl)CCCl)OCCCN1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 26.11 100
DMSO 26.11 100

Preparing Stock Solutions for Cyclophosphamide

The following data is based on the product molecular weight 261.09.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 3.83 mL 19.15 mL 38.30 mL
5 mM 0.77 mL 3.83 mL 7.66 mL
10 mM 0.38 mL 1.92 mL 3.83 mL
50 mM 0.08 mL 0.38 mL 0.77 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 7 publications citing the usage of this product.

  • Hengstler DNA strand breaks and DNA cross-links in peripheral mononuclear blood cells of ovarian cancer patients during chemotherapy with cyclophosphamide/carboplatin. Cancer Res.
  • Ren Inhibition of human aldehyde dehydrogenase 1 by the 4-hydroxycyclophosphamide degradation product acrolein. Drug Metab.Disp.
  • Gibson Regulation of BAX and BCL-2 expression in breast cancer cells by chemotherapy. Breast Cancer Research and Treatment 1999 PMID: 10481938

Product Documents for Cyclophosphamide

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cyclophosphamide

For research use only

⚠ WARNING: This product can expose you to chemicals including Cyclophosphamide (anhydrous), which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov

Citations for Cyclophosphamide

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