Cytochrome b5 type A Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-14923

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Canine, Feline

Predicted:

Bovine (91%), Canine (95%), Monkey (97%), Porcine (92%), Sheep (92%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cytochrome b5 type A Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytochrome b5 type A. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (97%), Chicken (82%), Mouse (88%), Rat (88%), Xenopus laevis (80%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytochrome b5 type A Antibody - BSA Free

Western Blot: Cytochrome b5 type A Antibody [NBP2-14923]

Western Blot: Cytochrome b5 type A Antibody [NBP2-14923]

Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Analysis of CYB5A expression in transfected 293T cell line by CYB5A polyclonal antibody. A: Non-transfected lysate. B: CYB5A transfected lysate. 12% SDS PAGE, antibody diluted at 1:500.
Cytochrome b5 type A Antibody

Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] -

Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Cytochrome b5 type A Antibody

Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] -

Western Blot: Cytochrome b5 type A Antibody [NBP2-14923] - Whole cell extract (30 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Cytochrome b5 type A Antibody

Immunocytochemistry/ Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-14923] -

Immunocytochemistry/ Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-14923] - Cytochrome b5 type A antibody detects Cytochrome b5 type A protein at cytoplasm by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytochrome b5 type A stained by Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500.
Blue: Hoechst 33342 staining.
Cytochrome b5 type A Antibody

Immunocytochemistry/ Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-14923] -

Immunocytochemistry/ Immunofluorescence: Cytochrome b5 type A Antibody [NBP2-14923] - Cytochrome b5 type A antibody detects Cytochrome b5 type A protein at cytoplasm by immunofluorescent analysis.
Sample: PG-4 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytochrome b5 type A stained by Cytochrome b5 type A antibody (NBP2-14923) diluted at 1:500.
Blue: Hoechst 33342 staining.

Applications for Cytochrome b5 type A Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytochrome b5 type A

The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

CYB5, cytochrome b-5, cytochrome b5 (microsomal), cytochrome b5 type A (microsomal), MCB5cytochrome b5, Microsomal cytochrome b5 type A, type 1 cyt-b5

Gene Symbol

CYB5A

UniProt

P00167 (Human)

Additional Cytochrome b5 type A Products

Product Documents for Cytochrome b5 type A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytochrome b5 type A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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