Cytokeratin 10 Antibody (DE-K10)

Novus Biologicals | Catalog # NBP2-47650

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Canine, Feline

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Hydrolysis Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # DE-K10
View all Cytokeratin 10 Primary Antibodies »

Product Specifications

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium (Uniprot: P13645)

Localization

Cytoplasmic

Marker

Suprabasal Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

56.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54402)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytokeratin 10 Antibody (DE-K10)

Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (DE-K10) [NBP2-47650]

Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (DE-K10) [NBP2-47650]

Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (DE-K10) [NBP2-47650] - Formalin-paraffin human Bladder Carcinoma stained with Cytokeratin 10 MAb (DE-K10).

Applications for Cytokeratin 10 Antibody (DE-K10)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 10

This gene encodes a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]

Alternate Names

BCIE, CK-10, CK10, EHK2A, EHK2B, K10, KPP

Gene Symbol

KRT10

UniProt

P13645 (Human)

Additional Cytokeratin 10 Products

Product Documents for Cytokeratin 10 Antibody (DE-K10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 10 Antibody (DE-K10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 10 Antibody (DE-K10)

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Protocols

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FAQs

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