Cytokeratin 17 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38536

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (92%), Rat (92%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cytokeratin 17 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SSRTSCRLSGGLGAGSCRLGSAGGL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cytokeratin 17 Antibody - BSA Free

Western Blot: Cytokeratin 17 Antibody [NBP2-38536]

Western Blot: Cytokeratin 17 Antibody [NBP2-38536]

Western Blot: Cytokeratin 17 Antibody [NBP2-38536] - Analysis in human cell lines HeLa and PC-3. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536]

Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536] - MYP3 rat urothelial cell line with CK17 (green) and DAPI (blue). Primary antibody at 1:200. Image from verified customer review.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536]

Immunocytochemistry/Immunofluorescence: Cytokeratin 17 Antibody [NBP2-38536] - UROtsa (rat urothelial cell line). CK17 in green, DAPI in blue. Secondary antibody: Goat Polyclonal Anti-Rabbit at 1:500. Image from verified customer review.

Applications for Cytokeratin 17 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04 - 0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reviewed Applications

Read 1 review rated 4 using NBP2-38536 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cytokeratin 17

Among the members of the cytokeratin (CK) subfamily of intermediate filament (IF) proteins, CK 17 is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. It has an unusual expression pattern in normal and diseased states and has potential importance in tumor diagnosis. CK 17 synthesis seems to be a marker of basal cell differentiation in complex epithelia and therefore indicative of a certain type of epithelial "stem cells" (1). It has been shown that CK 17 regulates cell growth through binding to the adaptor protein 14-3-3sigma. These findings reveal a new and unexpected role for the intermediate filament cytoskeleton in influencing cell growth and size by regulating protein synthesis (2). CK 17 is expressed in the nail bed, hair follicle, sebaceous glands, and other epidermal appendages (3).

Long Name

Cytokeratin 17/KRT17

Alternate Names

CK-17, cytokeratin-17, K17, keratin 17, type I, keratin, type I cytoskeletal 17, PCHC1

Gene Symbol

KRT17

UniProt

Q04695 (Human)

Additional Cytokeratin 17 Products

Product Documents for Cytokeratin 17 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cytokeratin 17 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cytokeratin 17 Antibody - BSA Free (1)

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Customer Images

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  • Cytokeratin 17 Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: bladder cell line
    Species: Rat
    Verified Customer | Posted 05/18/2018
    UROtsa (rat urothelial cell line). CK17 in green, DAPI in blue.
    1/200 dilution (would recommend higher in the future) with UROtsa rat urothelial cells. Secondary antibody of Goat Polyclonal Anti-Rabbit (Invitrogen) at 1/500 dilution.
    Cytokeratin 17 Antibody - BSA Free NBP2-38536

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Protocols

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