Cytokeratins are cytoskeletal intermediate filament proteins that are classified as type I (acidic) and type II (neutral to basic) and associate into heterotetramers.
Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
Novus Biologicals | Catalog # NBP2-48075
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075]](https://resources.rndsystems.com/images/products/Cytokeratin-18-Antibody-C-04-same-as-Ks18-04-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48075-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken (Negative), Equine, Feline, Ferret, Goat, Hamster, Monkey, Sheep, Xenopus (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # C-04 (same as Ks18.04)
Format
IHC-Prediluted
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Product Specifications
Immunogen
Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431
Reactivity Notes
Marmoset Monkey. Does not react with Chicken or Xenopus.
Localization
Cytoplasmic
Specificity
This monoclonal antibody reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075]
Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (C-04).![Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted Western Blot: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] -](https://resources.rndsystems.com/images/products/nbp2-48075_mouse-monoclonal-cytokeratin-18-antibody-c-04-same-as-ks18-04-ihc-prediluted-29520251637787.jpg "Western Blot: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] -")
Western Blot: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] -
Western Blot Analysis of human A) intestine and B) stomach lysate using Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted.![Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted Western Blot: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] -](https://resources.rndsystems.com/images/products/nbp2-48075_mouse-monoclonal-cytokeratin-18-antibody-c-04-same-as-ks18-04-ihc-prediluted-295202516351325.jpg "Western Blot: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] -")
Western Blot: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] -
Western Blot Analysis of HCT116 cell lysate using Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted.![Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted Flow Cytometry: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] -](https://resources.rndsystems.com/images/products/nbp2-48075_mouse-monoclonal-cytokeratin-18-antibody-c-04-same-as-ks18-04-ihc-prediluted-295202516414915.jpg "Flow Cytometry: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] -")
Flow Cytometry: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted [NBP2-48075] -
Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)Applications for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. Positive control: Tonsil. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 18
Alternate Names
CK-18, CYK18, Keratin 18, KRT18
Gene Symbol
KRT18
Additional Cytokeratin 18 Products
Product Documents for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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