Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48140

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KRT7/760

Format

IHC-Prediluted
View all Cytokeratin 7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cytokeratin 7 protein (Uniprot: P08729)

Localization

Cytoplasmic

Marker

Glandular and Transitional Epithelial Marker

Specificity

It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This monoclonal antibody is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This monoclonal antibody is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/760)
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140] - Human Lung Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/760)
Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140] - Human Endometrial Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/760)
Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140] -

Immunofluorescent staininf of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted Flow Cytometry: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140] -

Flow Cytometry: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140] -

Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells. Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted Western Blot: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140] -

Western Blot: Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted [NBP2-48140] -

Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted.

Applications for Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 7

Alternate Names

CK-7, CK7, K2C7, K7

Gene Symbol

KRT7

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted

There are currently no reviews for this product. Be the first to review Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted and earn rewards!

Have you used Cytokeratin 7 Antibody (KRT7/760) - IHC-Prediluted?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies