Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted

Novus Biologicals | Catalog # NBP2-48139

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Ferret (Negative), Mouse (Negative), Rabbit (Negative), Rat (Negative)

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # KRT7/903

Format

IHC-Prediluted
View all Cytokeratin 7 Primary Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Cytokeratin 7 protein (Uniprot: P08729)

Reactivity Notes

Does not react with Mouse, Rat, Ferret or Rabbit.

Localization

Cytoplasmic

Marker

Glandular and Transitional Epithelial Marker

Specificity

Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Antibody to cytokeratin is useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted [NBP2-48139]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted [NBP2-48139]

Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted [NBP2-48139] - Human Ovarian Carcinoma stained with Cytokeratin 7 Monoclonal Antibody (KRT7/903)
Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted [NBP2-48139] -

Immunocytochemistry/ Immunofluorescence: Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted [NBP2-48139] -

Immunofluorescence Analysis of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Redot.
Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted Flow Cytometry: Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted [NBP2-48139] -

Flow Cytometry: Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted [NBP2-48139] -

Flow Cytometric Analysis of trypsinized methanol-fixed HeLa cells using Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Applications for Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 12q13.13 Immunohistochemistry-Paraffin 1 - 2 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 7

Alternate Names

CK-7, CK7, K2C7, K7

Gene Symbol

KRT7

Additional Cytokeratin 7 Products

Product Documents for Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cytokeratin 7 Antibody (KRT7/903) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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