Cytokeratin 8/18 Antibody (5D3)

Novus Biologicals | Catalog # NB120-17139

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5D3
View all Cytokeratin 8/18 Primary Antibodies »

Product Specifications

Immunogen

Cytokeratins from the human breast carcinoma cell line MCF-7.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 11277418). Not yet tested in other species.

Localization

Cytoplasmic

Specificity

This antibody is specific to human cytokeratins 8 (52.5 kDa) and 18 (45 kDa). It stains simple and glandular epithelium. It can be useful for the identification of adenocarcinomas and most squamous cell carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Cytokeratin 8/18 Antibody (5D3)

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (5D3) [NB120-17139]

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (5D3) [NB120-17139]

Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (5D3) [NB120-17139] - Formalin fixed paraffin embedded human skin stained with Cytokeratin 8/18 antibody (NB120-17139).

Applications for Cytokeratin 8/18 Antibody (5D3)

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

Tissue culture supernatant

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 8/18

Cytokeratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with Cytokeratin 18 (type A; acidic subfamily of low molecular weight keratins).

Alternate Names

CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, Keratin-8, KO, Type-II keratin Kb8

Entrez Gene IDs

3856 (Human)

Gene Symbol

KRT8

Additional Cytokeratin 8/18 Products

Product Documents for Cytokeratin 8/18 Antibody (5D3)

Certificate of Analysis

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Product Specific Notices for Cytokeratin 8/18 Antibody (5D3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cytokeratin 8/18 Antibody (5D3)

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Protocols

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FAQs

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