Cytokeratin 8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16094

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Zebrafish

Cited:

Human

Predicted:

Rhesus Macaque (99%). Backed by our 100% Guarantee.

Applications

Validated:

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Whole-Mount, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cytokeratin 8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cytokeratin 8. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%), Porcine (83%), Bovine (84%), Canine (89%).

Localization

Cytoplasm

Marker

Epithelial Cell Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 8 Antibody - BSA Free

Western Blot: Cytokeratin 8 Antibody [NBP2-16094]

Western Blot: Cytokeratin 8 Antibody [NBP2-16094]

Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody [NBP2-16094] - Gastric ca, using Cytokeratin 8 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Cytokeratin 8 Antibody [NBP2-16094]

Western Blot: Cytokeratin 8 Antibody [NBP2-16094]

Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.
Cytokeratin 8 Antibody

Immunohistochemistry: Cytokeratin 8 Antibody [NBP2-16094] -

Immunohistochemistry: Cytokeratin 8 Antibody [NBP2-16094] - Cytokeratin 8 antibody detects Cytokeratin 8 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo.
Green: Cytokeratin 8 stained by Cytokeratin 8 antibody (NBP2-16094) diluted at 1:100.
Cytokeratin 8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] -

Cytokeratin 8 antibody detects Cytokeratin 8 protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cytokeratin 8 stained by Cytokeratin 8 antibody (NBP2-16094) diluted at 1:500.
Blue: Hoechst 33342 staining.
Cytokeratin 8 Antibody - BSA Free

Western Blot: Cytokeratin 8 Antibody [NBP2-16094] -

Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody (NBP2-16094) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Cytokeratin 8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry Whole-Mount

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000

Reviewed Applications

Read 1 review rated 5 using NBP2-16094 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cytokeratin 8

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. [provided by RefSeq]

Alternate Names

CARD2, CK8, EndoA, KRT8

Gene Symbol

KRT8

UniProt

P05787 (Human)

Additional Cytokeratin 8 Products

Product Documents for Cytokeratin 8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for Cytokeratin 8 Antibody - BSA Free

Customer Reviews for Cytokeratin 8 Antibody - BSA Free (1)

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  • Cytokeratin 8 Antibody
    Name: Alexandra Ambrico
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: mouse prostate
    Species: Mouse
    Verified Customer | Posted 10/06/2016
    Cytokeratin 8 Antibody - BSA Free NBP2-16094

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