DARC Antibody (18B11) - BSA Free

Novus Biologicals | Catalog # NBP3-26634

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 18B11 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from Human DARC [UniProt Q16570]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DARC Antibody (18B11) - BSA Free

DARC Antibody (18B11)

Flow Cytometry: DARC Antibody (18B11) [NBP3-26634] -

Flow Cytometry: DARC Antibody (18B11) [NBP3-26634] - Overlay Peak curve showing 293 cells stained with DARC Antibody (18B11) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for DARC Antibody (18B11) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: DARC

DARC is a non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.

Long Name

Duffy Antigen, Receptor For Chemokines

Alternate Names

ACKR1, CD234, Duffy Antigen, Fy Antigen, GPD, GpFy

Gene Symbol

ACKR1

Additional DARC Products

Product Documents for DARC Antibody (18B11) - BSA Free

Certificate of Analysis

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Product Specific Notices for DARC Antibody (18B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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