DBT Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89522

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DBT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VGKPLVDIETEALKDSEEDVVETPAVSHDEHTHQEIKGRKTLATPAVRRLAMENNIKLSEVVGSGKDGRILKEDILNYLEKQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DBT Antibody - BSA Free

DBT Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: DBT Antibody - BSA Free [NBP1-89522]

Immunocytochemistry/ Immunofluorescence: DBT Antibody - BSA Free [NBP1-89522]

Staining of human cell line A-431 shows localization to mitochondria.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon, liver, lymph node and pancreas using Anti-DBT antibody NBP1-89522 (A) shows similar protein distribution across tissues to independent antibody NBP1-85964 (B).
DBT Antibody - BSA Free Immunohistochemistry-Paraffin: DBT Antibody - BSA Free [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody - BSA Free [NBP1-89522]

Analysis in human kidney and pancreas tissues using NBP1-89522 antibody. Corresponding DBT RNA-seq data are presented for the same tissues.
DBT Antibody - BSA Free Immunohistochemistry-Paraffin: DBT Antibody - BSA Free [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody - BSA Free [NBP1-89522]

Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
DBT Antibody - BSA Free Immunohistochemistry-Paraffin: DBT Antibody - BSA Free [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody - BSA Free [NBP1-89522]

Staining of human pancreas shows weak to moderate cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human colon.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human prostate shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human liver.
Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody [NBP1-89522] - Staining of human lymph node.
DBT Antibody - BSA Free Immunohistochemistry-Paraffin: DBT Antibody - BSA Free [NBP1-89522]

Immunohistochemistry-Paraffin: DBT Antibody - BSA Free [NBP1-89522]

Staining of human testis shows weak to moderate cytoplasmic positivity in Leydig cells.
DBT Antibody - BSA Free Western Blot: DBT Antibody - BSA Free [NBP1-89522]

Western Blot: DBT Antibody - BSA Free [NBP1-89522]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
DBT Antibody - BSA Free Western Blot: DBT Antibody - BSA Free [NBP1-89522]

Western Blot: DBT Antibody - BSA Free [NBP1-89522]

Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)
Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)

Applications for DBT Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DBT

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Alternate Names

BCATE2BCKAD E2 subunit, BCKADE2, BCKAD-E2, branched chain acyltransferase, E2 component, Branched-chain alpha-keto acid dehydrogenase complex component E2, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto aciddehydrogenase complex, Dihydrolipoamide branched chain transacylase, dihydrolipoamide branched chain transacylase (E2 component of branched chainketo acid dehydrogenase complex; maple syrup urine disease), dihydrolipoamide branched chain transacylase E2, dihydrolipoyl transacylase, Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, E2, E2 component of branched chain alpha-keto acid dehydrogenase complex, E2B, EC 2.3.1.168, lipoamide acyltransferase component of branched-chain alpha-keto aciddehydrogenase complex, mitochondrial, lipoamide acyltransferase component of mitochondrial branched-chain alpha-ketoacid dehydrogenase complex, MGC9061, mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit(E2b)

Gene Symbol

DBT

Additional DBT Products

Product Documents for DBT Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for DBT Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for DBT Antibody - BSA Free

There are currently no reviews for this product. Be the first to review DBT Antibody - BSA Free and earn rewards!

Have you used DBT Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies