DCK Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-16108

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (95%), Rhesus Macaque (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all DCK Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human DCK. The exact sequence is proprietary.

Reactivity Notes

Chicken (82%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DCK Antibody - BSA Free

Western Blot: DCK Antibody [NBP2-16108]

Western Blot: DCK Antibody [NBP2-16108]

Western Blot: DCK Antibody [NBP2-16108] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: DCK Antibody [NBP2-16108]

Western Blot: DCK Antibody [NBP2-16108]

Western Blot: DCK Antibody [NBP2-16108] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Immunohistochemistry-Paraffin: DCK Antibody [NBP2-16108]

Immunohistochemistry-Paraffin: DCK Antibody [NBP2-16108]

Immunohistochemistry-Paraffin: DCK Antibody [NBP2-16108] - Rat lung. DCK stained by DCK antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Western Blot: DCK Antibody [NBP2-16108]

Western Blot: DCK Antibody [NBP2-16108]

Western Blot: DCK Antibody [NBP2-16108] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody diluted at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: DCK Antibody [NBP2-16108]

Western Blot: DCK Antibody [NBP2-16108]

Western Blot: DCK Antibody [NBP2-16108] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract 12% SDS-PAGE DCK antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: DCK Antibody [NBP2-16108]

Immunohistochemistry-Paraffin: DCK Antibody [NBP2-16108]

Immunohistochemistry-Paraffin: DCK Antibody [NBP2-16108] - Mouse hind brain. DCK antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunoprecipitation: DCK Antibody [NBP2-16108]

Immunoprecipitation: DCK Antibody [NBP2-16108]

Immunoprecipitation: DCK Antibody [NBP2-16108] - Immunoprecipitation of DCK protein from 293T whole cell extracts using 5 ug of DCK antibody. Western blot analysis was performed using DCK antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
DCK Antibody

Western Blot: DCK Antibody [NBP2-16108] -

Western Blot: DCK Antibody [NBP2-16108] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with DCK antibody (NBP2-16108) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
DCK Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DCK Antibody [NBP2-16108] -

DCK antibody detects DCK protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DCK protein stained by DCK antibody (NBP2-16108) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
Scale bar = 10 um.

Applications for DCK Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:1000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DCK

Deoxycytidine kinase (DCK) is required for the phosphorylation of several deoxyribonucleosides and their nucleoside analogs. Deficiency of DCK is associated with resistance to antiviral and anticancer chemotherapeutic agents. Conversely, increased deoxycytidine kinase activity is associated with increased activation of these compounds to cytotoxic nucleoside triphosphate derivatives. DCK is clinically important because of its relationship to drug resistance and sensitivity. [provided by RefSeq]

Long Name

Deoxycytidine kinase

Alternate Names

EC 2.7.1.113, EC 2.7.1.74, EC 2.7.1.76

Gene Symbol

DCK

UniProt

P27707 (Human)

Additional DCK Products

Product Documents for DCK Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for DCK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for DCK Antibody - BSA Free

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Protocols

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FAQs

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